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LOXL1-AS1 Contributes to Non-Small Cell Lung Cancer Progression by Regulating miR-3128/RHOXF2 Axis.
Limin Zhao1, Xuefei Zhang2, Huannan Guo1
1Department of Oncology, General Hospital of Heilongjiang General Administration of Agriculture and Reclamation, Harbin 150088, People's Republic of China.
Oncotargets and Therapy
|July 9, 2020
Summary
Long non-coding RNA LOXL1-AS1 promotes non-small cell lung cancer (NSCLC) progression. It regulates the miR-3128/RHOXF2 axis, suggesting LOXL1-AS1 as a potential diagnostic and therapeutic target for NSCLC.
Area of Science:
- Molecular Oncology
- Cancer Biology
Background:
- Non-small cell lung cancer (NSCLC) remains a leading cause of cancer-related mortality worldwide.
- Understanding the molecular mechanisms underlying NSCLC progression is crucial for developing effective therapies.
Purpose of the Study:
- To elucidate the molecular mechanism of long non-coding RNA (lncRNA) LOXL1-AS1 in the progression of non-small cell lung cancer (NSCLC).
Main Methods:
- Gene expression analysis using quantitative real-time PCR (qRT-PCR) in NSCLC cell lines and tissues.
- Bioinformatic prediction of target genes using miRDB and TargetScan7.
- Functional assays including CCK8 for proliferation and Transwell assays for migration and invasion.
- Luciferase, pull-down, and rescue assays to validate molecular interactions.
Main Results:
- LncRNA LOXL1-AS1 expression was significantly upregulated in NSCLC tissues and cells, correlating with advanced stages and metastasis.
- Knockdown of LOXL1-AS1 inhibited NSCLC cell proliferation, migration, and invasion.
- LOXL1-AS1 was found to negatively regulate miR-3128, which in turn suppressed RHOXF2 expression.
- Rescue experiments confirmed the role of the miR-3128/RHOXF2 axis in mediating LOXL1-AS1's effects on NSCLC progression.
Conclusions:
- LncRNA LOXL1-AS1 promotes NSCLC progression through the regulation of the miR-3128/RHOXF2 axis.
- LOXL1-AS1 represents a potential novel therapeutic target for the diagnosis and treatment of NSCLC.
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