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Related Experiment Video

Updated: Dec 15, 2025

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
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Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

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Proteomics profiling asthma induced-lysine acetylation.

Xin-Ming Su1, Yuan Ren1, Meng-Lu Li1

  • 1Department of Pulmonary and Critical Care Medicine, Institute of Respiratory Diseases, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China.

EXCLI Journal
|July 9, 2020
PubMed
Summary
This summary is machine-generated.

This study reveals that protein acetylation, particularly histone acetylation, is crucial for controlling asthma. Targeting the novel histone acetylation motif -KAXXK- offers new therapeutic strategies for this chronic inflammatory disease.

Keywords:
HDACiacetylationacetylproteomeasthma

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Area of Science:

  • Biochemistry
  • Immunology
  • Molecular Biology

Background:

  • Asthma is a chronic inflammatory airway disease with no definitive cure.
  • Protein acetylation, especially histone acetylation, is implicated in asthma pathogenesis.
  • Histone deacetylation inhibitors (HDACi) show therapeutic potential in asthma.

Purpose of the Study:

  • To investigate the role of protein acetylation in asthma using proteome and acetylproteome analysis.
  • To identify key regulatory mechanisms and potential therapeutic targets in asthma.
  • To explore the efficacy of HDAC inhibitors in an asthma model.

Main Methods:

  • Created an ovalbumin-induced asthma mouse model.
  • Performed comprehensive proteome and acetylproteome analysis on lung tissues.
  • Utilized bioinformatics for pathway and motif analysis, and tested HDAC inhibitors.

Main Results:

  • Identified 5346 proteins and 581 acetylation sites; 154 proteins and 68 peptides were significantly altered in asthma.
  • Bioinformatics revealed altered biological processes, pathways, and protein groups.
  • Discovered a novel histone acetylation motif, -KAXXK-, linked to asthma regulation.

Conclusions:

  • Asthma involves significant proteome and acetylation dynamics.
  • Protein lysine acetylation, regulated by the -KAXXK- motif, plays a key role in asthma.
  • These findings suggest new therapeutic targets for asthma treatment.