Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

674
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
674

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Testosterone protects from metabolic syndrome-associated lung dysfunction in a high-fat diet rabbit model.

Endocrinology·2026
Same author

Spatial and temporal variability of supraglacial algae on an Alpine glacier (Forni Glacier, Italy).

Scientific reports·2026
Same author

Mechanical de-skewing enables high-resolution imaging of thin tissue slices with a mesoSPIM light-sheet microscope.

Biomedical optics express·2026
Same author

β<sub>3</sub>-adrenoceptor agonism exerts lung protection in a rat model of bronchopulmonary dysplasia.

British journal of pharmacology·2026
Same author

Versatile and comprehensive hyperspectral imaging tool for molecular neuronavigation: a case study on cerebral gliomas.

Journal of biomedical optics·2025
Same author

A Label-Free Hyperspectral Imaging Device for Ex Vivo Characterization and Grading of Meningioma Tissues.

Journal of biophotonics·2025

Related Experiment Video

Updated: Dec 15, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.0K

Fast multi-directional DSLM for confocal detection without striping artifacts.

Pietro Ricci1,2, Giuseppe Sancataldo1,3, Vladislav Gavryusev1,2

  • 1European Laboratory for Non-Linear Spectroscopy, Sesto Fiorentino, 50019, Italy.

Biomedical Optics Express
|July 9, 2020
PubMed
Summary
This summary is machine-generated.

Light-sheet fluorescence microscopy (LSFM) can create artifacts. This study introduces a multi-directional illumination method using an acousto-optical deflector to reduce these artifacts, improving 3D brain imaging quality.

More Related Videos

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.2K
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

485

Related Experiment Videos

Last Updated: Dec 15, 2025

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.0K
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.2K
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

485

Area of Science:

  • Neuroscience
  • Microscopy
  • Optical Engineering

Background:

  • Light-sheet fluorescence microscopy (LSFM) is crucial for 3D neuroscience imaging, offering optical sectioning and reduced photodamage.
  • LSFM images can suffer from uneven illumination and striping artifacts due to light scattering and absorption.
  • Existing methods struggle to fully correct these artifacts while maintaining image quality.

Purpose of the Study:

  • To develop and validate an optical solution for reducing artifacts in LSFM imaging.
  • To implement a fast multi-directional illumination system using an acousto-optical deflector (AOD).
  • To assess the compatibility of this system with digital scanned laser light-sheet fluorescence microscopy (DSLM) and confocal detection.

Main Methods:

  • Utilized an acousto-optical deflector (AOD) to create a fast, multi-directional, pivoting elliptical-Gaussian beam for illumination.
  • Integrated the pivoting beam system with digital scanned laser light-sheet fluorescence microscopy (DSLM) and confocal detection.
  • Acquired fluorescence signals from mouse brain tissue, comparing pivoting versus static beam illumination and analyzing image quality metrics.

Main Results:

  • Demonstrated real-time suppression of shadow artifacts and striping.
  • Quantified significant reduction in striping artifacts compared to traditional static illumination.
  • Confirmed preservation of confocal detection advantages, including high image contrast.

Conclusions:

  • The developed pivoting illumination system effectively reduces artifacts in LSFM.
  • This method enhances 3D imaging quality in neuroscience applications without compromising image contrast.
  • The acousto-optical deflector-based approach offers a robust solution for improved LSFM performance.