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Data on nucleoid-associated proteins isolated from Mycoplasma Gallisepticum in different growth phases

I Zubov Aleksandr1, A Semashko Tatiana1, V Evsyutina Daria1

  • 1Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.

Data in Brief
|July 9, 2020
PubMed

Insights

This study reveals dynamic proteomic changes in Mycoplasma gallisepticum nucleoids during growth phases. Understanding these shifts in DNA-binding proteins aids in comprehending chromosome organization and stress adaptation.

Area of Science:

  • Microbiology
  • Proteomics
  • Molecular Biology

Background:

  • Mycoplasma gallisepticum (MG) is a minimal bacterium with a reduced genome and no cell wall.
  • Limited DNA-binding proteins and transcription factors in MG necessitate understanding nucleoid dynamics.
  • Nucleoid protein profiles are crucial for MG's functioning, chromosome organization, and stress adaptation.

Purpose of the Study:

  • To investigate dynamic proteomic changes in MG nucleoids across different growth phases.
  • To identify proteins involved in MG chromosome organization and stress response.
  • To provide insights into the functional mechanisms of MG nucleoids.

Main Methods:

  • Quantitative proteomic analysis of MG nucleoids.
  • Synchronous cell culture in logarithmic and stationary phases with a starvation period.
  • LC-MS/MS analysis using DDA and DIA modes, with data processed by MaxQuant and Skyline.

Main Results:

  • Significant alterations in the nucleoid proteomic profile were observed between logarithmic and stationary growth phases.
  • Identification of specific DNA-binding proteins and other nucleoid-associated proteins that change in abundance.
  • The study provides a quantitative dataset of nucleoid protein dynamics in MG.

Conclusions:

  • The proteomic landscape of the MG nucleoid undergoes significant dynamic changes correlating with growth phases.
  • These proteomic shifts are likely key to regulating chromosome organization and adapting to environmental stress.
  • This research offers a foundation for further studies into MG's molecular mechanisms.
Keywords:
Data-independent acquisitionMycoplasma gallisepticumNucleoidNucleoid-associated proteinsProteomic analysisSynchronous growth

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