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A new chromogenic substrate for assay and detection of alpha-amylase
P Biely1, D Mislovicová, O Markovic
1Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Analytical Biochemistry
|July 1, 1988
Abstract:
A new soluble chromogenic substrate for alpha-amylase was prepared by coupling partially hydrolyzed starch with a dye, Ostazin brilliant red H-3B. The substrate is precipitable from buffered solutions with ethanol and is equally suitable for assay of alpha-amylase, detection of separated alpha-amylase isoenzymes in gels, and selection of microbial producers of the enzyme.