Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Histone Modification02:32

Histone Modification

15.5K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
15.5K
Histone Modification02:32

Histone Modification

4.1K
4.1K
The Nucleosome Core Particle01:12

The Nucleosome Core Particle

2.0K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
2.0K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.1K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metab8D: a metabolic regulome network from multiomics and machine learning.

Communications biology·2026
Same author

Modeling Microbiome Modulation of Tumor Metabolic Networks to Predict Synergistic Therapies.

bioRxiv : the preprint server for biology·2026
Same author

AI-driven drug discovery and repurposing using multi-omics for myocardial infarction and heart failure.

Exploration of medicine·2026
Same author

Reprogramming SREBP1-dependent lipogenesis and inflammation in high-risk breast with licochalcone A: A novel path to cancer prevention.

International journal of cancer·2025
Same author

Rapid prediction of thermodynamically destabilizing tyrosine phosphorylations in cancers.

bioRxiv : the preprint server for biology·2025
Same author

Multi-Cohort Exploration of Repetitive Element Transcription and DNA Methylation in Human Steatotic Liver Disease.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Dec 15, 2025

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.5K

Nutrient Sensing by Histone Marks: Reading the Metabolic Histone Code Using Tracing, Omics, and Modeling.

Scott E Campit1, Alia Meliki2, Neil A Youngson3,4,5

  • 1Program in Chemical Biology, University of Michigan, Ann Arbor, MI, 48109, USA.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|July 9, 2020
PubMed
Summary

Metabolites influence the epigenome through histone modifications, impacting health and disease. Systems biology approaches like flux tracing can reveal how nutrient levels shape epigenetic marks in cells.

More Related Videos

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

30.2K
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.6K

Related Experiment Videos

Last Updated: Dec 15, 2025

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.5K
Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
11:02

Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis

Published on: May 17, 2016

30.2K
The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
24:02

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

18.6K

Area of Science:

  • Biochemistry
  • Epigenetics
  • Systems Biology

Background:

  • Metabolites are substrates for histone modifications, linking metabolic state to the epigenome.
  • Metabolomics and proteomics reveal nutrient availability's impact on histone modifications.
  • Metabolism-epigenome crosstalk is crucial for development, immunity, and cancer.

Purpose of the Study:

  • To address challenges in understanding metabolism-epigenome interactions.
  • To explore systems biology methods for uncovering new metabolic-epigenetic links.
  • To elucidate how nutrients shape the epigenome in diverse cells.

Main Methods:

  • Review of recent applications of systems biology.
  • Discussion of flux tracing and metabolic modeling.
  • Integration of metabolomics and proteomics data.

Main Results:

  • Systems biology offers powerful tools to study metabolism-epigenome interactions.
  • Flux tracing and metabolic modeling can identify novel metabolic-epigenetic links.
  • Nutrient-dependent epigenetic regulation is a key area for future research.

Conclusions:

  • Systems biology is essential for deciphering the complex interplay between metabolism and epigenetics.
  • Understanding these interactions can provide insights into disease mechanisms.
  • Further research can leverage these approaches to uncover fundamental biological processes.