Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Contribution of astrocytic calcium signaling to auditory hypersensitivity in a mouse model of fragile X syndrome.

Neurobiology of disease·2026
Same author

Enhanced Primary Motor Cortex Astrocyte Calcium Signaling With Motor Learning.

Neural plasticity·2026
Same author

Patchy striatonigral neurons modulate locomotor vigor in response to environmental valence.

eLife·2025
Same author

Activity of Human-Specific Interlaminar Astrocytes in a Chimeric Mouse Model of Fragile X Syndrome.

International journal of molecular sciences·2025
Same author

Poor Correlation Between Inflammatory and Brain Injury Biomarkers After Neonatal Intraventricular Hemorrhage: A Pilot Study.

World neurosurgery·2025
Same author

Patchy Striatonigral Neurons Modulate Locomotor Vigor in Response to Environmental Valence.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Dec 15, 2025

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice
10:13

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

Published on: August 12, 2014

13.8K

Modeling human-specific interlaminar astrocytes in the mouse cerebral cortex.

Ragunathan Padmashri1, Baiyan Ren2, Braden Oldham1

  • 1Department of Neurological Sciences, University of Nebraska Medical Center, Omaha, Nebraska, USA.

The Journal of Comparative Neurology
|July 9, 2020
PubMed
Summary

Human interlaminar astrocytes, unique to primate brains, were modeled in mice. This breakthrough allows studying their function and role in neurological diseases.

Keywords:
astrocytescortexiPSCinterlaminarprimate

More Related Videos

3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue
08:57

3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue

Published on: July 16, 2021

6.9K
Isolation and Culture of Mouse Cortical Astrocytes
11:25

Isolation and Culture of Mouse Cortical Astrocytes

Published on: January 19, 2013

93.7K

Related Experiment Videos

Last Updated: Dec 15, 2025

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice
10:13

Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice

Published on: August 12, 2014

13.8K
3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue
08:57

3D Bioprinting of Murine Cortical Astrocytes for Engineering Neural-Like Tissue

Published on: July 16, 2021

6.9K
Isolation and Culture of Mouse Cortical Astrocytes
11:25

Isolation and Culture of Mouse Cortical Astrocytes

Published on: January 19, 2013

93.7K

Area of Science:

  • Neuroscience
  • Glial cell biology
  • Astrocyte heterogeneity

Background:

  • Astrocytes regulate brain development and homeostasis.
  • Human astrocytes exhibit unique interlaminar astrocytes in the cerebral cortex.
  • The function of these primate-specific astrocytes is poorly understood due to limited models.

Purpose of the Study:

  • To model human interlaminar astrocytes in a living organism.
  • To investigate the functional properties of human interlaminar astrocytes.
  • To explore their role in neuronal circuit regulation and neurological diseases.

Main Methods:

  • Human induced pluripotent stem cells (hiPSCs) were differentiated into astrocytes.
  • These human astrocytes were engrafted into the mouse cortex, creating humanized glial chimeric mice.
  • This model allows for in vivo study of human astrocyte function.

Main Results:

  • Successfully modeled human interlaminar astrocytes in a chimeric mouse brain.
  • Established a novel platform for studying human astrocyte-mouse neuron interactions.
  • Opened avenues for investigating astrocyte dysfunction in neurological conditions.

Conclusions:

  • Humanized glial chimeric mice provide a viable model for studying primate-specific astrocytes.
  • This model facilitates research into neuron-glial interactions.
  • It offers a new tool for understanding and potentially treating neurological diseases involving astrocytes.