Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement fixation by solid phase immune complexes. Reduced capacity in SLE sera.

G Baatrup1, H Jonsson, A Sjöholm

  • 1Institute of Medical Microbiology, University of Odense, Denmark.

Journal of Clinical & Laboratory Immunology
|June 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cryosurgery versus curettage for intraepidermal carcinoma: A randomized controlled trial.

Journal of the European Academy of Dermatology and Venereology : JEADV·2023
Same author

Interobserver agreement on landmark and flexure identification in colon capsule endoscopy.

Techniques in coloproctology·2023
Same author

Edge artificial intelligence wireless video capsule endoscopy.

Scientific reports·2022
Same author

Curettage vs. cryosurgery for superficial basal cell carcinoma: a prospective, randomised and controlled trial.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022
Same author

Point-of-care ultrasound of the heart and lungs in patients with respiratory failure: a pragmatic randomized controlled multicenter trial.

Scandinavian journal of trauma, resuscitation and emergency medicine·2021
Same author

Colon capsule endoscopy versus CT colonography after incomplete colonoscopy. Application of artificial intelligence algorithms to identify complete colonic investigations.

United European gastroenterology journal·2020
Same journal

Policy and science of FMD control: the stakeholders' contribution to decision making. A call for integrated animal disease management.

Journal of clinical & laboratory immunology·2006
Same journal

An informative case of Graves' disease with implications for schizophrenia.

Journal of clinical & laboratory immunology·2006
Same journal

Increase of circulating CD8+CD57+ lymphocytes after measles infection but not after measles vaccination.

Journal of clinical & laboratory immunology·2006
Same journal

Review: immunomodulatory activity of pregnancy-associated plasma protein-A.

Journal of clinical & laboratory immunology·2004
Same journal

Antibodies to Acinetobacter bacteria and bovine brain peptides, measured in bovine spongiform encephalopathy (BSE) in an attempt to develop an ante-mortem test.

Journal of clinical & laboratory immunology·2004
Same journal

Principles of autoimmune disease: pathogenesis, genetics and specific immunotherapy.

Journal of clinical & laboratory immunology·2004
See all related articles

This study introduces a new ELISA to measure complement function by assessing its ability to bind to immune complexes. This assay effectively tracks complement component uptake, offering insights into disease activity, particularly in systemic lupus erythematosus patients.

Area of Science:

  • Immunology
  • Biochemistry
  • Clinical Chemistry

Background:

  • Complement system plays a crucial role in immune response and immune complex clearance.
  • Dysfunction of the complement system is implicated in various autoimmune diseases, including systemic lupus erythematosus (SLE).
  • Assessing complement function is vital for understanding disease pathogenesis and monitoring treatment efficacy.

Purpose of the Study:

  • To develop and validate an enzyme-linked immunosorbent assay (ELISA) for evaluating complement function.
  • To assess the capacity of serum to support the fixation of complement components onto solid-phase immune complexes (IC).
  • To investigate the utility of this assay in monitoring disease activity in patients with SLE.

Main Methods:

  • Microplates were coated with aggregated bovine serum albumin (BSA) and rabbit anti-BSA IgG to form solid-phase IC.

Related Experiment Videos

  • Human serum was reacted with the solid-phase IC.
  • Uptake of complement components (C3b, C4b, properdin) was quantified using specific biotinylated antibodies and an enzyme-linked detection system.

    • Main Results:

    • The ELISA demonstrated varying levels of reduced complement component uptake (C4b, C3b, properdin) in SLE patients' sera.
    • Reduced uptake of C3b and C4b, and C3b and properdin, correlated with high disease activity.
    • Assay results indicated that complement component uptake, IC solubilization, and C1q binding IC were more consistent indicators of SLE disease activity than circulating complement component concentrations.

    Conclusions:

    • The developed ELISA is a valuable tool for assessing complement function and its role in SLE.
    • Complement component uptake assays provide a more sensitive measure of complement activation and disease activity in SLE compared to measuring circulating levels.
    • This assay has the potential to aid in the clinical management and monitoring of patients with SLE and other complement-mediated disorders.