Related Experiment Video
Updated: Dec 15, 2025
Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications: Manufacturing and Characterization
Published on: July 3, 2018
Fabrication and characterization of ultrathin spin-coated poly(L-lactic acid) films suitable for cell attachment and
Viktoryia Kulikouskaya1, Ihnat Chyshankou1, Sergei Pinchuk2
1Laboratory of Micro- and Nanostructured Systems, Institute of Chemistry of New Materials of National Academy of Sciences of Belarus, Minsk, Belarus.
Abstract:
In the present study, ultrathin poly(L-lactic acid) (PLLA) films were fabricated using the spin-coating technique. Physicochemical properties of the formed materials, including their morphology, thickness, transparency, and contact angle, have been studied. We determined that the morphology of PLLA films could be regulated by changing the polymer concentration and humidity. By altering the humidity, microporous and flat PLLA films can be fabricated. The obtained samples were subsequently used for culturing mesenchymal stem cells and fibroblasts. It has been determined that cells effectively adhered to prepared films and formed on them a monolayer culture with high viability. It has been shown that PLLA films are suitable for the entrapment of curcumin (up to 12.1 μm cm-2) and provide its sustained release in solutions isotonic to blood plasma. The obtained PLLA films appear to be prospective materials for potential application in regenerative medicine as part of cell-containing tissue engineered dressings for chronic wound treatment.
