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Modulation of some human mononuclear cells activities by procaine
A R Jurjus1, D Jarrush-Saadeh, C Nassar
1Department of Human Morphology, Faculty of Medicine, American University of Beirut, Lebanon.
Middle East Journal of Anaesthesiology
|June 1, 1988
Summary
Procaine, a local anesthetic, was found to inhibit key functions of human immune cells, including T-lymphocyte and B-lymphocyte rosette formation, monocyte phagocytosis, and lymphocyte proliferation, suggesting it acts as an immunoregulator.
Area of Science:
- Immunology
- Pharmacology
- Cell Biology
Background:
- Procaine is a local anesthetic known for its membrane-active properties.
- Human peripheral blood mononuclear cells (PBMCs) include lymphocytes and monocytes, crucial for immune responses.
- Understanding drug effects on immune cell function is vital for therapeutic applications.
Purpose of the Study:
- To investigate the in vitro effects of procaine on the functional activities of human lymphocytes and monocytes.
- To determine if procaine modulates immune cell interactions and proliferation.
Main Methods:
- Studied the effects of procaine on spontaneous E-rosette and EAC-rosette formation in human lymphocytes.
- Assessed procaine's impact on monocyte phagocytosis of latex particles.
- Evaluated procaine's influence on lymphocyte proliferation in allogeneic mixed leukocyte cultures.
- Observed morphological changes in procaine-treated cells.
Main Results:
- Procaine inhibited T-lymphocyte and B-lymphocyte rosette formation.
- Procaine reduced the phagocytic activity of monocytes.
- Procaine suppressed lymphocyte proliferation in mixed leukocyte cultures.
- Morphological analysis showed an increased cytoplasmic rim in procaine-treated cells.
Conclusions:
- Procaine demonstrated inhibitory effects on multiple functional activities of human peripheral blood mononuclear cells.
- Procaine may act as a non-specific immunoregulator, modulating immune cell functions.
- These findings suggest potential immunomodulatory roles for procaine beyond local anesthesia.