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Erythropoietin changes the globin program of an interleukin 3-dependent multipotential cell line
T Enver1, B Nakamoto, J Karlinsey
1Division of Medical Genetics, University of Washington, Seattle 98195.
Summary
Adult hematopoietic stem cells can express embryonic globin, suggesting developmental plasticity. This finding indicates that globin gene programs may not be permanently altered during development.
Area of Science:
- Hematology
- Developmental Biology
- Stem Cell Biology
Background:
- B6SUtA cells are adult murine hematopoietic stem cells with multipotent properties.
- Erythroid differentiation involves sequential expression of embryonic and adult globin genes.
Purpose of the Study:
- To analyze globin gene expression during erythroid differentiation of B6SUtA cells.
- To compare globin expression in B6SUtA cells with embryonic erythroid progenitors.
Main Methods:
- Culturing B6SUtA cells in suspension and clonal cultures.
- Analyzing globin expression with and without erythropoietin.
- Comparing B6SUtA cell globin programs with mouse embryonic erythroid progenitors.
Main Results:
- B6SUtA cells express predominantly embryonic globin without erythropoietin.
- Erythropoietin promotes maturation and preferential adult globin expression.
- Single B6SUtA progenitors can express both embryonic and adult globin.
- B6SUtA globin programs resemble early embryonic erythroid progenitors.
Conclusions:
- Adult hematopoietic stem cells retain the capacity to express embryonic globin.
- The globin locus may not be irreversibly modified during development.
- Adult erythroid progenitors can transiently express primitive globin programs.