Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 15, 2025

Isolation and Characterization of Single Cells from Zebrafish Embryos
09:25

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

15.0K

Comparative analysis of single-cell transcriptomics in human and Zebrafish oocytes.

Handan Can1, Sree K Chanumolu1, Elena Gonzalez-Muñoz2,3

  • 1Department of Electrical and Computer Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.

BMC Genomics
|July 10, 2020
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum proteomics in paediatric inflammatory bowel disease from a case-control study: biomarker discovery and ulcerative colitis-Crohn's disease differentiation.

EBioMedicine·2026
Same author

3D Glioblastoma Molecular Responses to Carbon Dot-Delivered Riluzole Probed by Synchrotron FTIR.

Analytical chemistry·2026
Same author

Top-Down Proteomics of Zebrafish Brain Regions Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry.

Journal of proteome research·2026
Same author

Uncovering zebrafish embryonic proteome dynamics across 16 time points during the first 24 hours of development.

bioRxiv : the preprint server for biology·2026
Same author

Development of Cells Repository of <i>Betta</i> Species: A Tool for Genetic Conservation and Biotechnological Advancement.

Animals : an open access journal from MDPI·2026
Same author

Top-Down Proteomics Study of Aging and Sexual Dimorphism of Zebrafish Brains.

Journal of mass spectrometry : JMS·2025

Zebrafish and human oocytes share highly expressed orthologous genes involved in essential functions. This finding supports zebrafish as a valid model for studying human oocyte biology.

Area of Science:

  • Developmental Biology
  • Comparative Genomics
  • Oocyte Biology

Background:

  • Zebrafish are widely used in developmental biology.
  • Direct comparison of zebrafish and human oocyte transcriptomes is understudied.
  • Investigating gene expression similarities between zebrafish and human oocytes is significant.

Purpose of the Study:

  • To compare zebrafish and human oocyte transcriptomes.
  • To assess the conservation of gene expression in oocytes between zebrafish and humans.
  • To determine if gene sequence similarity translates to expression level similarity.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) of zebrafish oocytes.
  • Comparative analysis with human oocyte scRNA-seq data.
Keywords:
OocyteOrthologyRNA-seqTranscriptomeZebrafish

More Related Videos

Author Spotlight: Enhanced Method for Isolating Pure Zebrafish Stage I Oocytes
05:12

Author Spotlight: Enhanced Method for Isolating Pure Zebrafish Stage I Oocytes

Published on: July 26, 2024

1.3K
Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish
11:35

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

Published on: April 18, 2021

8.2K

Related Experiment Videos

Last Updated: Dec 15, 2025

Isolation and Characterization of Single Cells from Zebrafish Embryos
09:25

Isolation and Characterization of Single Cells from Zebrafish Embryos

Published on: March 12, 2016

15.0K
Author Spotlight: Enhanced Method for Isolating Pure Zebrafish Stage I Oocytes
05:12

Author Spotlight: Enhanced Method for Isolating Pure Zebrafish Stage I Oocytes

Published on: July 26, 2024

1.3K
Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish
11:35

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

Published on: April 18, 2021

8.2K
  • Orthology analysis and systems biology approaches.

    • Main Results:

    • High consistency between human oocyte studies.
    • Significant concordance in expression of orthologous genes between zebrafish and humans.
    • Higher orthology percentage for highly expressed genes compared to lowly expressed genes.
    • Overlapping enriched pathways and GO terms in highly expressed genes.
    • Commonly overexpressed orthologous genes are crucial for oocyte function.

    Conclusions:

    • Orthologous genes are highly expressed concurrently in zebrafish and human oocytes.
    • These shared genes belong to similar functional categories.
    • Zebrafish serve as a valid model organism for human oocyte research.