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Isolation and Characterization of Single Cells from Zebrafish Embryos
Published on: March 12, 2016
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Comparative analysis of single-cell transcriptomics in human and Zebrafish oocytes
Handan Can1, Sree K Chanumolu1, Elena Gonzalez-Muñoz2,3
1Department of Electrical and Computer Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
BMC Genomics
|July 10, 2020
Summary
Zebrafish and human oocytes share highly expressed orthologous genes involved in essential functions. This finding supports zebrafish as a valid model for studying human oocyte biology.
Area of Science:
- Developmental Biology
- Comparative Genomics
- Oocyte Biology
Background:
- Zebrafish are widely used in developmental biology.
- Direct comparison of zebrafish and human oocyte transcriptomes is understudied.
- Investigating gene expression similarities between zebrafish and human oocytes is significant.
Purpose of the Study:
- To compare zebrafish and human oocyte transcriptomes.
- To assess the conservation of gene expression in oocytes between zebrafish and humans.
- To determine if gene sequence similarity translates to expression level similarity.
Main Methods:
- Single-cell RNA sequencing (scRNA-seq) of zebrafish oocytes.
- Comparative analysis with human oocyte scRNA-seq data.
- Orthology analysis and systems biology approaches.
Main Results:
- High consistency between human oocyte studies.
- Significant concordance in expression of orthologous genes between zebrafish and humans.
- Higher orthology percentage for highly expressed genes compared to lowly expressed genes.
- Overlapping enriched pathways and GO terms in highly expressed genes.
- Commonly overexpressed orthologous genes are crucial for oocyte function.
Conclusions:
- Orthologous genes are highly expressed concurrently in zebrafish and human oocytes.
- These shared genes belong to similar functional categories.
- Zebrafish serve as a valid model organism for human oocyte research.
