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Updated: Dec 15, 2025
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
Genome-wide identification of methylated CpG sites in nongenital cutaneous warts
Laith N Al-Eitan1,2, Mansour A Alghamdi3,4, Amneh H Tarkhan5
1Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid, 22110, Jordan. lneitan@just.edu.jo.
Background:
Low-risk HPV infection has not been the subject of epigenetic investigation. The present study was carried out in order to investigate the methylation status of CpG sites in non-genital cutaneous warts.
Methods:
Genomic DNA was extracted from 24 paired epidermal samples of warts and normal skin. DNA samples were bisulfite converted and underwent genome-wide methylation profiling using the Infinium MethylationEPIC BeadChip Kit.
Results:
From a total of 844,234 CpG sites, 56,960 and 43,040 CpG sites were found to be hypo- and hypermethylated, respectively, in non-genital cutaneous warts. The most differentially methylated CpG sites in warts were located within the C10orf26, FAM83H-AS1, ZNF644, LINC00702, GSAP, STAT5A, HDAC4, NCALD, and EXOC4 genes.
Conclusion:
Non-genital cutaneous warts exhibit a unique CpG methylation signature.
