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New sensitive bioassay for sulbactam in bovine tissues
G H Keeler1, J P Stankewich, A E Girard
1Quality Control Division, Pfizer Inc., Groton, Connecticut 06340.
Antimicrobial Agents and Chemotherapy
|September 1, 1988
Summary
A new bioassay accurately quantifies sulbactam in animal tissues at low levels. This sensitive method, unaffected by ampicillin, is valuable for tissue depletion studies.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Biochemistry
Background:
- Sulbactam is an important beta-lactamase inhibitor.
- Accurate quantification of sulbactam in biological matrices is crucial for pharmacokinetic and residue studies.
- Existing bioassays may lack sensitivity or specificity.
Purpose of the Study:
- To develop and validate a highly sensitive quantitative bioassay for sulbactam.
- To determine the applicability of the assay to various bovine tissues.
- To assess the assay's performance, including accuracy and precision.
Main Methods:
- A quantitative cylinder plate bioassay was developed.
- The assay utilizes the inhibition of cell-free beta-lactamase by sulbactam.
- Bovine muscle, fat, kidney, and liver tissues were analyzed.
Main Results:
- The bioassay can detect sulbactam concentrations as low as 0.01 microgram/g.
- The method demonstrated high accuracy and precision.
- The assay is unaffected by ampicillin, even at high concentrations.
Conclusions:
- A sensitive and specific bioassay for sulbactam in bovine tissues has been established.
- This method is suitable for tissue depletion studies and may be applicable to human samples.
- The assay's robustness and sensitivity offer advantages over previous methods.
