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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Dec 15, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

COVID-19: Current Trends in Invitro Diagnostics.

R Arun Krishnan¹, Rhema Elizabeth Thomas¹, Ajaikumar Sukumaran¹

¹R&D Reagent Department, Agappe Diagnostics Limited, Cochin, Kerala India.

Indian Journal of Clinical Biochemistry : IJCB

|July 10, 2020

Summary

This summary is machine-generated.

Early detection of COVID-19 is crucial. This review discusses in vitro diagnostic methods, highlighting nucleic acid tests and immunoassays for accurate SARS-CoV-2 diagnosis and patient management.

Keywords:

CLIA ELISA Lateral flow immunoassay Loop mediated isothermal amplification Reverse transcriptase PCR SARS-CoV-2

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Last Updated: Dec 15, 2025

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Area of Science:

Virology
Infectious Diseases
Diagnostic Medicine

Background:

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) causes the COVID-19 pandemic.
COVID-19 has spread globally at an unprecedented rate.
No specific antiviral treatments or vaccines are currently available for COVID-19.

Purpose of the Study:

To review current in vitro diagnostic methods for COVID-19.
To discuss the role of these diagnostic tools in early detection and prognosis.

Main Methods:

Review of existing literature on in vitro diagnostic techniques for SARS-CoV-2.
Analysis of the reliability and application of different diagnostic platforms.

Main Results:

Nucleic acid-based polymerase chain reaction (PCR) is the established reliable diagnostic platform.
Antigen and antibody detection immunoassays serve as effective screening tests.
These methods are vital for early detection and prognosis in COVID-19 patient management.

Conclusions:

Accurate and timely diagnosis is essential to control the spread of COVID-19.
Nucleic acid tests and immunoassays are key tools in the global diagnostic strategy.
Continued development and application of diagnostic methods are critical for managing the pandemic.