Related Experiment Video
Updated: Oct 14, 2026

Murine Model of CD40-activation of B cells
Published on: March 5, 2010
Lipopolysaccharide-induced murine B cell proliferation: a role of protein kinase C
S Gupta1, S Gollapudi, B Vayuvegula
1Department of Medicine, University of California, Irvine 92717.
Abstract:
Lipopolysaccharide (LPS)-induced murine B cell proliferation was blocked by 1-(5-isoquinoline-sulfonyl)-2-methylpiperazine dihydrochloride (H7), an inhibitor of protein kinase C (PKC), in a dose- and time-dependent manner. The maximum inhibition of B cell proliferation was observed when H7 was added at the initiation of cultures. H7-induced inhibition was prolonged and irreversible. Furthermore, pretreatment of B cells with phorbol myristate acetate ester, a process that degrades membrane-associated PKC, rendered them unresponsive to LPS. These data strongly suggest that the activation of PKC is one of the mechanisms of LPS-induced murine B cell proliferation.
Related Concept Videos
The JAK-STAT Signaling Pathway
PI3K/mTOR/AKT Signaling Pathway
cAMP-dependent Protein Kinase Pathways
IP3/DAG Signaling Pathway
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

