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Related Concept Videos

Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

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Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
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Single Nucleotide Polymorphisms-SNPs01:05

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A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
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Related Experiment Video

Updated: Dec 15, 2025

Lung CT Segmentation to Identify Consolidations and Ground Glass Areas for Quantitative Assesment of SARS-CoV Pneumonia
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A Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse Transcriptase-Polymerase Chain Reaction and

Zhengtu Li1, Yinhu Li2, Lingdan Chen1

  • 1State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
|July 10, 2020
PubMed
Summary

A case of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia was diagnosed despite negative RT-PCR tests. Nanopore sequencing and antibody detection identified SARS-CoV-2, highlighting limitations of RT-PCR with virus variation.

Keywords:
SARS-CoV-2nanopore sequencingpneumoniaroutine RT-PCRvirus variation

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Area of Science:

  • Infectious Diseases
  • Virology
  • Molecular Diagnostics

Background:

  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a significant global health concern.
  • Current diagnosis relies heavily on reverse transcriptase-polymerase chain reaction (RT-PCR) for SARS-CoV-2 detection.

Purpose of the Study:

  • To report a confirmed case of SARS-CoV-2 pneumonia with a negative RT-PCR result.
  • To investigate alternative diagnostic methods for SARS-CoV-2 infection when RT-PCR is inconclusive.

Main Methods:

  • A case study of a patient with SARS-CoV-2 pneumonia presenting with negative RT-PCR results.
  • Utilized nanopore sequencing for pathogen identification.
  • Employed SARS-CoV-2 antibody detection for confirmation.

Main Results:

  • The patient was diagnosed with SARS-CoV-2 pneumonia through nanopore sequencing and antibody detection, despite initial negative RT-PCR.
  • Variations in the ORF and NP genes of SARS-CoV-2 were identified in this case.
  • This case underscores the potential for false-negative RT-PCR results, particularly in the presence of viral genetic variation.

Conclusions:

  • Routine RT-PCR may yield false-negative results for SARS-CoV-2 pneumonia, especially when viral mutations are present.
  • Nanopore pathogen sequencing and antibody detection offer effective complementary diagnostic tools for clinical settings.
  • These advanced methods are crucial for accurate diagnosis and management of infectious diseases with evolving pathogens.