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Illuminating Cellular Biochemistry: Fluorogenic Chemogenetic Biosensors for Biological Imaging
Fanny Broch1, Arnaud Gautier1,2
1Sorbonne Université, École normale supérieure, PSL University, CNRS Laboratoire des biomolécules, LBM, 75005, Paris, France.
Chempluschem
|July 10, 2020
Summary
New fluorogenic reporters offer advanced cellular imaging capabilities. These systems utilize protein or RNA tags with fluorogenic dyes for versatile, high-performance biosensor design, enhancing optical recording of cellular processes.
Area of Science:
- Cellular and Molecular Imaging
- Biophysics
- Biotechnology
Background:
- Cellular activity relies on precise spatiotemporal regulation of molecular components.
- Optical recording using fluorescent biosensors is crucial for studying cell physiology.
- Existing biosensor technologies have limitations in reporter diversity and functionality.
Purpose of the Study:
- To review the recent advancements in fluorogenic systems for biosensor design.
- To highlight the potential of novel fluorogenic reporters in cellular imaging.
- To explore new perspectives for creating innovative and advanced biosensors.
Main Methods:
- Review of engineered photoreceptors, self-labeling proteins, and non-covalent aptamers/protein tags.
- Analysis of fluorogenic reporters comprising protein/RNA tags complexing with fluorogenic dyes.
- Discussion of the properties and applications of these novel reporter systems.
Main Results:
- Fluorogenic reporters offer expanded capabilities including far-red to near-infrared emission.
- These systems provide oxygen independence and versatile color options for biosensors.
- Engineered tags and dyes enable precise optical recording of biological events.
Conclusions:
- Fluorogenic systems represent a significant expansion in the toolkit for biosensor development.
- These reporters enable the design of unique and innovative biosensors for cellular imaging.
- The technology pushes the boundaries of optical recording in cell biology research.
