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Human non-interferon macrophage activating factor: biological and biochemical characterization
J C Lee1, A M Badger, W J Johnson
1Department of Immunology & Antiinfective Therapy, Smith Kline & French Laboratories, Philadelphia, PA 19101.
A HTLV-1 transformed T cell line has been demonstrated to constitutively produce and secrete a lymphokine with macrophage activating properties. This lymphokine was biologically and biochemically distinct from interferon gamma, the interleukins, the colony stimulating factors and lymphotoxin. In vitro treatment of human monocytes with the partially purified lymphokine resulted in the acquisition of microbicidal and tumoricidal activities. The activity was associated with a protein with an apparent molecular weight of 55,000 daltons and an isoelectric point of 5.5. The potential for the efficacy of such a lymphokine in activating macrophages in vivo provides aspirations for its use in the therapy/prevention of opportunistic infections in immunocompromised hosts.
A HTLV-1 transformed T cell line has been demonstrated to constitutively produce and secrete a lymphokine with macrophage activating properties. This lymphokine was biologically and biochemically distinct from interferon gamma, the interleukins, the colony stimulating factors and lymphotoxin. In vitro treatment of human monocytes with the partially purified lymphokine resulted in the acquisition of microbicidal and tumoricidal activities. The activity was associated with a protein with an apparent molecular weight of 55,000 daltons and an isoelectric point of 5.5. The potential for the efficacy of such a lymphokine in activating macrophages in vivo provides aspirations for its use in the therapy/prevention of opportunistic infections in immunocompromised hosts.