Related Experiment Videos
Dendritic cells from rat lung are potent accessory cells.
C L Rochester1, E M Goodell, J K Stoltenborg
1Medical Research Institute, Mary Imogene Bassett Hospital, Cooperstown, NY 13326.
The American Review of Respiratory Disease
|July 1, 1988
Summary
Pulmonary dendritic cells (DC-P) are potent accessory cells that enhance lymphocyte proliferation. These cells, found in low-density rat pulmonary cells, are crucial for immune responses.
Area of Science:
- Immunology
- Cell Biology
- Pulmonary Research
Background:
- Accessory cells are essential for lymphocyte proliferation.
- The role of pulmonary accessory cells in immune responses requires further investigation.
Purpose of the Study:
- To identify and characterize accessory cells in rat lungs.
- To determine the role of pulmonary accessory cells in periodate-induced lymphocyte proliferation.
Main Methods:
- Rat lungs were digested to obtain pulmonary cells (PC).
- PC were separated into high-density (HD-PC) and low-density (LD-PC) fractions using a density gradient.
- Accessory activity of different cell fractions was assessed using periodate-treated lymphocytes.
Main Results:
- Low-density pulmonary cells (LD-PC) exhibited the most accessory activity.
- Pulmonary dendritic cells (DC-P), isolated from LD-PC, significantly enhanced lymphocyte proliferation.
- Macrophages within the LD-PC fraction suppressed lymphocyte proliferation.
Conclusions:
- Pulmonary dendritic cells are potent accessory cells for periodate-induced lymphocyte proliferation.
- The separation and purification of pulmonary dendritic cells are crucial for understanding their immune function.