Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

6.6K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
6.6K
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

5.7K
Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
5.7K
Combined Effects of Drugs: Antagonism01:30

Combined Effects of Drugs: Antagonism

11.3K
The combined effects of drugs can result in various interactions, of which an important type is antagonism. Antagonism is a mechanism where one drug inhibits or counteracts the effects of another drug. Antagonism can occur through various means, including receptor binding, allosteric modulation, functional interaction, chemical reactions, and pharmacokinetic processes.
The most common type is receptor antagonism, where one drug acts as an antagonist to block the effects of another drug by...
11.3K
Genetic Screens02:46

Genetic Screens

5.5K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.5K
Agonism and Antagonism: Quantification01:14

Agonism and Antagonism: Quantification

856
When drugs are administered, they can elicit either an agonist or antagonist effect on the body. Agonism occurs when a drug activates a specific receptor, triggering a biological response. On the other hand, antagonism happens when a drug binds to the same receptors but blocks their activation, thereby preventing a biological response.
To quantify these effects, researchers use a dose-response curve, which provides valuable information about the potency and efficacy of a drug. Potency refers to...
856
Drug Discovery: Overview01:26

Drug Discovery: Overview

10.7K
Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
10.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design of poly(vinyl pyrrolidone) and poly(ethylene glycol) microneedle arrays for delivering glycosaminoglycan, chondroitin sulfate, and hyaluronic acid.

Journal of biomaterials science. Polymer edition·2024
Same author

Evaluation of the Possible Contribution of Various Regulatory Genes to Determination of Carpel Number as a Potential Mechanism for Optimal Agricultural Yield.

International journal of molecular sciences·2022
Same author

Isolation and characterization of the most abundant rhizobacterial species associated with Vuralia turcica (Fabaceae: Papilionoideae).

Anais da Academia Brasileira de Ciencias·2022
Same author

ZnO and MXenes as electrode materials for supercapacitor devices.

Beilstein journal of nanotechnology·2021
Same author

Author Correction: Antibiotic susceptibility signatures identify potential antimicrobial targets in the Acinetobacter baumannii cell envelope.

Nature communications·2020
Same author

Silencing of survivin and cyclin B1 through siRNA-loaded arginine modified calcium phosphate nanoparticles for non-small-cell lung cancer therapy.

Colloids and surfaces. B, Biointerfaces·2020

Related Experiment Video

Updated: Dec 15, 2025

Diagonal Method to Measure Synergy Among Any Number of Drugs
12:08

Diagonal Method to Measure Synergy Among Any Number of Drugs

Published on: June 21, 2018

19.3K

Guided screen for synergistic three-drug combinations.

Melike Cokol-Cakmak1, Selim Cetiner1, Nurdan Erdem1

  • 1Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey.

Plos One
|July 10, 2020
PubMed
Summary

Discovering effective three-drug combinations is challenging. This study introduces a guided screen using pairwise interactions to predict and validate synergistic drug combinations, reducing screening by five-fold.

More Related Videos

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method
07:51

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method

Published on: May 21, 2018

12.4K
Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations
11:15

Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations

Published on: July 24, 2021

5.2K

Related Experiment Videos

Last Updated: Dec 15, 2025

Diagonal Method to Measure Synergy Among Any Number of Drugs
12:08

Diagonal Method to Measure Synergy Among Any Number of Drugs

Published on: June 21, 2018

19.3K
High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method
07:51

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method

Published on: May 21, 2018

12.4K
Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations
11:15

Quadruple-Checkerboard: A Modification of the Three-Dimensional Checkerboard for Studying Drug Combinations

Published on: July 24, 2021

5.2K

Area of Science:

  • Microbiology
  • Pharmacology
  • Computational Biology

Background:

  • High-order drug combinations are crucial in oncology and infectious diseases to combat resistance and enhance efficacy.
  • The vast number of potential combinations poses a significant challenge for identifying synergistic drug therapies.
  • Discovering synergistic three-drug combinations requires efficient screening strategies.

Purpose of the Study:

  • To develop and validate a guided screening approach for identifying synergistic three-drug combinations.
  • To reduce the experimental burden in discovering potent multi-drug therapies.
  • To apply the method to identify synergistic combinations against Erwinia amylovora.

Main Methods:

  • Experimental measurement of all pairwise drug interactions using checkerboard and diagonal methods.
  • Prediction of three-drug synergy/antagonism scores by averaging pairwise interaction scores.
  • Experimental validation of predicted synergistic three-drug combinations.

Main Results:

  • Agreement between checkerboard and diagonal methods for pairwise synergy measurements.
  • Successful prediction of three-drug synergy scores based on pairwise data.
  • Validation of 35 predicted three-drug combinations.
  • A five-fold reduction in screening size to identify top synergistic combinations.

Conclusions:

  • The guided screening strategy effectively identifies synergistic three-drug combinations.
  • Averaging pairwise synergy scores is a reliable predictor of three-drug interactions.
  • This approach significantly enhances the efficiency of discovering multi-drug synergies.