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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Related Experiment Video

Updated: Dec 15, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
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Local resolution estimates of cryoEM reconstructions.

J L Vilas1, J B Heymann2, H D Tagare1

  • 1Department of Biomedical Engineering, Yale University, New Haven, United States.

Current Opinion in Structural Biology
|July 10, 2020
PubMed
Summary
This summary is machine-generated.

Local resolution in cryo-electron microscopy (cryoEM) aids structural biology by revealing sample heterogeneity and flexibility. This review clarifies local resolution concepts and offers best practices to prevent misinterpretation of cryoEM data.

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Area of Science:

  • Structural Biology
  • Biophysics
  • Biochemistry

Background:

  • Cryo-electron microscopy (cryoEM) has rapidly advanced, achieving quasi-atomic resolutions in structural biology.
  • Despite progress, accurate resolution estimation remains a critical challenge in cryoEM.
  • New computational and methodological developments necessitate updated approaches to resolution assessment.

Purpose of the Study:

  • To review the concept of local resolution in cryoEM.
  • To discuss current algorithms for local resolution estimation.
  • To provide best practices for interpreting local resolution maps and avoid misinterpretations.

Main Methods:

  • Review of existing literature on local resolution estimation algorithms.
  • Comparative analysis of different local resolution measurement approaches.
  • Synthesis of best practices for data interpretation.

Main Results:

  • Local resolution analysis offers insights into sample heterogeneity, flexibility, and angular errors.
  • Current local resolution methods are not uniformly defined, leading to potential interpretation issues.
  • A set of guidelines is proposed to ensure accurate interpretation of local resolution data.

Conclusions:

  • Local resolution is a valuable tool for interpreting cryoEM reconstructions, aiding in modeling.
  • Standardization and careful application of local resolution methods are crucial.
  • Adherence to best practices will enhance the reliability and utility of cryoEM structural data.