Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of Implant Abutment-Soft Tissue Attachment Using 3D Tissue-Engineered Oral Mucosa: A Systematic Review.

International journal of dentistry·2026
Same author

Differential Attachment of Engineered Oral Soft Tissues to Implant Surfaces.

Dentistry journal·2026
Same author

A New Classification System to Determine Posterior Mandible Morphology for Implant Therapy.

International journal of dentistry·2026
Same author

Biological Evaluation of Oral Care Products Using 3D Tissue-Engineered In Vitro Models of Plaque-Induced Gingivitis.

Dentistry journal·2024
Same author

3D Printing of Dental Prostheses: Current and Emerging Applications.

Journal of composites science·2024
Same author

Effect of Different Silane Coupling Agents on the Bond Strength between Hydrogen Peroxide-Etched Epoxy-Based- Fiber-Reinforced Post and Composite Resin Core.

Dentistry journal·2023

Related Experiment Video

Updated: Dec 15, 2025

Oral Biofilm Formation on Different Materials for Dental Implants
11:19

Oral Biofilm Formation on Different Materials for Dental Implants

Published on: June 24, 2018

12.0K

Implant Soft-Tissue Attachment Using 3D Oral Mucosal Models-A Pilot Study.

Emilia Barker1, Lina AlQobaly1, Zahab Shaikh1

  • 1School of Clinical Dentistry, University of Sheffield, Western Bank, Sheffield S10 2TN, UK.

Dentistry Journal
|July 11, 2020
PubMed
Summary

This study shows greater soft tissue attachment to titanium-zirconium alloy with sand-blasted and acid-etched (SLA) surfaces compared to other materials in an inflamed oral mucosal model. This 3D model aids in testing implant soft tissue integration.

Keywords:
implantoral mucosasoft tissue attachmenttissue engineeringtitaniumzirconia

More Related Videos

Digital Hybrid Model Preparation for Virtual Planning of Reconstructive Dentoalveolar Surgical Procedures
09:10

Digital Hybrid Model Preparation for Virtual Planning of Reconstructive Dentoalveolar Surgical Procedures

Published on: August 5, 2021

2.1K
Effects of Mechanical Methods Used in Peri-implantitis Treatment on Implant Surface Decontamination and Roughness
06:36

Effects of Mechanical Methods Used in Peri-implantitis Treatment on Implant Surface Decontamination and Roughness

Published on: March 14, 2025

649

Related Experiment Videos

Last Updated: Dec 15, 2025

Oral Biofilm Formation on Different Materials for Dental Implants
11:19

Oral Biofilm Formation on Different Materials for Dental Implants

Published on: June 24, 2018

12.0K
Digital Hybrid Model Preparation for Virtual Planning of Reconstructive Dentoalveolar Surgical Procedures
09:10

Digital Hybrid Model Preparation for Virtual Planning of Reconstructive Dentoalveolar Surgical Procedures

Published on: August 5, 2021

2.1K
Effects of Mechanical Methods Used in Peri-implantitis Treatment on Implant Surface Decontamination and Roughness
06:36

Effects of Mechanical Methods Used in Peri-implantitis Treatment on Implant Surface Decontamination and Roughness

Published on: March 14, 2025

649

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Oral Biology

Background:

  • Understanding soft-tissue integration with dental implants is crucial for long-term success.
  • Inflammation significantly impacts the host response to implant materials.
  • Existing in vitro models may not fully replicate the complex oral environment.

Purpose of the Study:

  • To evaluate soft-tissue attachment to various implant surfaces (metal, ceramic, polymer) using an inflamed, 3D, tissue-engineered human oral mucosal model.
  • To employ multiple biological assays for quantitative and qualitative assessment of tissue-implant interactions.

Main Methods:

  • Engineered full-thickness, 3D human oral mucosal models using oral fibroblasts, keratinocytes, and monocytes.
  • Inserted implant materials: sand-blasted and acid-etched (SLA) and machined (M) titanium-zirconium alloy (TiZr), ceramic (ZrO2), and polyether ether ketone (PEEK).
  • Simulated inflammation using lipopolysaccharide (LPS) and tumor necrosis factor-alpha (TNF-α); assessed attachment via histology, pull-test (PrestoBlue assay), and scanning electron microscopy (SEM).

Main Results:

  • Successfully engineered inflamed, 3D oral mucosal models with integrated implants.
  • The TiZr-SLA surface demonstrated statistically significant higher tissue viability and attachment compared to TiZr-M, ceramic, and PEEK surfaces (p < 0.05).
  • SEM confirmed epithelial cell attachment across different implant surfaces.

Conclusions:

  • The developed inflamed, 3D oral mucosal model is a viable in vitro system for studying implant soft-tissue attachment.
  • TiZr-SLA surfaces exhibit superior soft tissue attachment compared to TiZr-M, ceramic, and PEEK in this model.