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Related Experiment Video

Updated: Dec 15, 2025

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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TopoWellPlate: A Well-Plate-Based Screening Platform to Study Cell-Surface Topography Interactions.

Nick R M Beijer1, Aliaksei S Vasilevich1, Bayram Pilavci1

  • 1Department of Cell Biology Inspired Tissue Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, Maastricht, 6229, ER, The Netherlands.

Advanced Biosystems
|July 11, 2020
PubMed
Summary

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The Floor-Ceiling-Chip, or 2 × 2D = Pseudo-3D-Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell-Adhesive Protein Micropatterns.

Advanced healthcare materials·2026

A novel TopoWellPlate system enables high-throughput screening of biomaterial interactions using a well-based strategy. This approach allows for diverse cellular responses to be analyzed with standard biochemical assays, advancing mechanotransduction studies.

Area of Science:

  • Biomaterial Engineering
  • Cell Biology
  • High-Throughput Screening

Background:

  • High-throughput approaches are crucial for studying cell-material interactions in biomaterial engineering.
  • Current chip-based material libraries often rely on immunofluorescence, limiting compatibility with standard biochemical assays.

Purpose of the Study:

  • To introduce a novel well-based strategy for preparing material libraries compatible with standard biochemical assays.
  • To present the TopoWellPlate, a system for high-throughput analysis of cell-surface topography interactions.

Main Methods:

  • Fabrication of topographically enhanced polystyrene films via a multistep cleanroom process.
  • Integration of these films into bottomless 96-well plates using thermal bonding to create the TopoWellPlate.
Keywords:
cell-material interactionhigh-throughput screeninghuman mesenchymal stem cellssurface topographywell plate-based

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  • Assessment of bone marrow-derived human mesenchymal stem cells' metabolic activity on the TopoWellPlate.

    • Main Results:

    • Identification of 87 uniquely defined bioactive surface topographies from a larger library.
    • Demonstration that these topographies induce a wide variety of cellular morphologies.
    • Measurement of a 2.5-fold difference in metabolic activity per cell, validating the TopoWellPlate's screening functionality.

    Conclusions:

    • The TopoWellPlate system facilitates high-throughput screening of cell-surface topography interactions.
    • This platform enables the use of standardized molecular assays to investigate biomaterial-induced mechanotransduction.
    • The system supports the discovery of novel biomaterials with specific cellular responses.