Related Experiment Video

Updated: Dec 15, 2025

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

2.2K

Albumin and calcium reference interval using healthy individuals and a data-mining approach

N Jassam1, A Luvai2, D Narayanan3

  • 1Department of Clinical Biochemistry, Harrogate Foundation Trust, Harrogate, UK.

Annals of Clinical Biochemistry
|July 11, 2020
PubMed

Insights

Harmonizing calcium reference intervals shows promise across UK platforms, but albumin intervals exhibit significant variation, challenging common reference ranges. Further investigation is needed for albumin standardization.

Area of Science:

  • Clinical Chemistry
  • Laboratory Medicine
  • Analytical Chemistry

Background:

  • Harmonization of reference intervals is recommended for analytes with established calibration and traceability.
  • The UK Pathology Harmony initiative has recently harmonized reference intervals for calcium and albumin.
  • This study aimed to determine reference intervals for calcium and albumin on common UK analytical platforms.

Purpose of the Study:

  • To assess the feasibility of harmonizing reference intervals for calcium and albumin.
  • To compare newly determined reference intervals with existing UK Pathology Harmony intervals.
  • To evaluate the performance of different analytical platforms in relation to these harmonized intervals.

Main Methods:

  • Recruited a prospective reference population (n=299) following IFCC CRIDL criteria.
  • Collected a second, indirect population from 14 primary care settings.
  • Measured analytes using Roche, Abbott, Beckman, and Siemens analytical platforms and methods.

Main Results:

  • Calcium reference intervals largely agreed with UK Pathology Harmony intervals (2.2-2.6 mmol/L) across three of four platforms.
  • BCG methods yielded higher reference limits for both calcium and albumin compared to BCP methods.
  • Indirect study revealed age-related variations in reference intervals, up to 14% for older populations.

Conclusions:

  • Calcium reference intervals demonstrate potential for harmonization across different analytical platforms.
  • Albumin reference intervals showed substantial variation, indicating that a common reference interval is not currently supported.
  • Analytical platform and method choice significantly impact reference intervals, particularly for albumin.
Abstract

Keywords:
Calciumanalytical systems, reference intervals, albumin, standardisation

More Related Videos

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis07:45

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis

Published on: February 9, 2021

3.9K
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli12:19

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Published on: June 27, 2015

11.4K

Related Concept Videos

Skeleton and Calcium Homeostasis01:21

Skeleton and Calcium Homeostasis

Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
5.5K
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area.
98
Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance01:25

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance

In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance...
107
Serum Studies: Renal Function Tests01:24

Serum Studies: Renal Function Tests

Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.
269
Bioequivalence Data: Statistical Interpretation01:16

Bioequivalence Data: Statistical Interpretation

Body:The statistical interpretation of bioequivalence data is a significant aspect of pharmaceutical research. Bioequivalence refers to the absence of any significant difference in the rate and extent to which the active ingredient in pharmaceutical products becomes available at the site of drug action when administered at the same molar dose under similar conditions. This helps determine if different drug products have similar absorption rates, ensuring their interchangeability.Statistical...
119