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Long non-coding RNA CCDC144NL-AS1 sponges miR-143-3p and regulates MAP3K7 by acting as a competing endogenous RNA in

Hao Fan1, Yugang Ge1, Xiang Ma1

  • 1Department of General Surgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China.

Cell Death & Disease
|July 11, 2020
PubMed

Insights

Long non-coding RNA CCDC144NL-AS1 promotes gastric cancer (GC) progression by regulating the miR-143-3p/MAP3K7 axis. This finding highlights CCDC144NL-AS1 as a potential diagnostic and therapeutic target for GC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GC) remains a leading cause of cancer mortality globally.
  • Long non-coding RNAs (lncRNAs) are implicated in cancer development, but their roles in GC require further elucidation.
  • The lncRNA CCDC144NL-AS1 has not been previously studied in the context of GC.

Purpose of the Study:

  • To investigate the functional role and molecular mechanism of lncRNA CCDC144NL-AS1 in gastric cancer.
  • To determine if CCDC144NL-AS1 can serve as a prognostic biomarker for GC.
  • To explore the potential of the CCDC144NL-AS1/miR-143-3p/MAP3K7 axis as a therapeutic target in GC.

Main Methods:

  • Quantitative real-time PCR to assess CCDC144NL-AS1 expression in GC tissues.
  • In vivo and in vitro functional assays (proliferation, migration, invasion, apoptosis).
  • Mechanism investigation involving RNA immunoprecipitation and Western blotting to confirm the ceRNA activity and target interactions.

Main Results:

  • CCDC144NL-AS1 was significantly upregulated in GC tissues and associated with larger tumor size and lymph node metastasis.
  • CCDC144NL-AS1 overexpression promoted GC cell proliferation, invasion, and migration while inhibiting apoptosis.
  • CCDC144NL-AS1 functions as a competing endogenous RNA (ceRNA) by sponging miR-143-3p, thereby upregulating MAP3K7 expression.

Conclusions:

  • The CCDC144NL-AS1/miR-143-3p/MAP3K7 axis plays a crucial oncogenic role in gastric cancer progression.
  • CCDC144NL-AS1 is a potential independent predictor of poor prognosis in GC patients.
  • Targeting the CCDC144NL-AS1 axis offers a promising strategy for novel GC diagnostics and therapeutics.

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