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Related Experiment Video

Updated: Dec 15, 2025

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

Gene Expression in Torn Rotator Cuff Tendons Determined by RNA Sequencing.

Robert Z Tashjian¹, Ian Lock¹, Erin K Granger¹

¹Department of Orthopaedics, University of Utah School of Medicine, Salt Lake City, Utah, USA.

Orthopaedic Journal of Sports Medicine

|July 11, 2020

Summary

Hypoxia may initiate rotator cuff tears, with smaller tears showing improved healing. Larger tears exhibit inflammation and poorer healing outcomes, suggesting distinct molecular pathways in rotator cuff disease progression.

Keywords:

RNA sequencing angiogenesis endothelial genetics hypoxia inflammation rotator cuff

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Last Updated: Dec 15, 2025

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

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Published on: December 12, 2016

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Published on: June 9, 2018

Area of Science:

Orthopedics
Molecular Biology
Biochemistry

Background:

The exact cause of rotator cuff tears is unknown, despite theories of multifactorial origins and genetic predisposition.
The biochemical underpinnings of rotator cuff tearing require further elucidation.

Purpose of the Study:

To investigate gene expression profiles in torn rotator cuff tendon tissue using RNA sequencing.
To identify molecular differences between healthy and torn rotator cuff tendons.

Main Methods:

RNA sequencing was performed on supraspinatus tendon biopsies from patients with rotator cuff tears and healthy controls.
Differential gene expression analysis was conducted between tear and control groups.
Unsupervised hierarchical clustering was used for secondary analysis to identify distinct molecular subtypes of tears.

Main Results:

Over 3000 genes showed differential expression between torn and control rotator cuff tendons.
Tears were categorized into two clusters: smaller, less retracted tears with increased hypoxia-related gene expression, and larger, more retracted tears with markers of chronic inflammation and angiogenesis.
Tears in the larger, more retracted group exhibited significantly worse healing rates (0%) compared to smaller tears (89%).

Conclusions:

Hypoxia may be an initial factor in rotator cuff tear development.
As tears enlarge, chronic inflammation and angiogenic processes may become dominant.
Gene expression profiling shows potential for predicting rotator cuff repair healing outcomes.