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Morinda citrifolia (Noni) Juice Suppresses A549 Human Lung Cancer Cells via Inhibiting AKT/Nuclear Factor-κ B

Ling-di Ma1,2, Gui-Bin Lin3, Lu-Bo Yang4

  • 1Laboratory Center, the Third People's Hospital of Huizhou, Affiliated Hospital of Guangzhou Medical University, Huizhou, Guangdong Province, 516002, China. lingdimawsh@126.com.

Chinese Journal of Integrative Medicine
|July 11, 2020
PubMed
Abstract

Insights

Noni juice demonstrates anti-tumor effects by inhibiting lung cancer cell proliferation and migration. It also promotes apoptosis and regulates the AKT/NF-κB signaling pathway, offering potential therapeutic benefits.

Area of Science:

  • Oncology
  • Pharmacology
  • Cell Biology

Background:

  • Morinda citrifolia (noni) is traditionally used for medicinal purposes.
  • Lung cancer remains a leading cause of cancer-related mortality worldwide.
  • Understanding the molecular mechanisms of natural compounds is crucial for developing novel cancer therapies.

Purpose of the Study:

  • To elucidate the anti-tumor mechanism of noni juice in A549 human lung cancer cells.
  • To investigate the effect of noni juice on cancer cell proliferation, apoptosis, invasion, and migration.
  • To determine the impact of noni juice on the AKT/nuclear factor-κB (NF-κB) signaling pathway.

Main Methods:

  • In vitro assays (MTT, CCK-8, colony formation, Annexin V/PI, transwell, scratch) were used to assess noni juice effects on A549 cells.
  • Immunoblotting and immunohistochemistry were employed to analyze protein expression and signaling pathway activity.
  • In vivo studies involved inoculating A549 cells in nude mice and administering noni juice to evaluate tumor growth and related markers.

Main Results:

  • Noni juice significantly inhibited A549 cell proliferation, migration, and invasion in vitro.
  • Noni juice induced apoptosis in A549 cells and suppressed tumor formation in vivo.
  • Noni juice treatment downregulated the phosphorylation of AKT, p50, and STAT3, and modulated apoptosis-related proteins (Ki67, PCNA, Bcl-2, caspase-3) in tumor tissues.
  • Noni juice restrained the activity of the AKT/NF-κB signaling pathway in both in vitro and in vivo models.

Conclusions:

  • Noni juice exhibits potent anti-cancer properties against A549 lung cancer cells.
  • The anti-tumor effects are mediated through the inhibition of cell proliferation, invasion, and migration, coupled with the induction of apoptosis.
  • Noni juice exerts its effects, at least in part, by regulating the AKT/NF-κB signaling pathway.
Keywords:
AKT/NF-κ B signaling pathwayChinese medicineMorinda citrifolialung cancer cellnoni juice

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