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CPEB3 regulates the proliferation and apoptosis of bovine cumulus cells

Ying Wang1, Cheng-Zhen Chen1, Xu-Huang Fu1

  • 1Department of Laboratory Animals, Jilin Provincial Key Laboratory of Animal Model, Jilin University, Changchun, Jilin, China.

Animal Science Journal = Nihon Chikusan Gakkaiho
|July 11, 2020
PubMed

Insights

Cytoplasmic polyadenylation element-binding protein 3 (CPEB3) regulates bovine cumulus cell functions. Inhibiting CPEB3 altered cell cycle, increased proliferation, and decreased apoptosis, impacting cell physiology.

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Cytoplasmic polyadenylation element-binding protein 3 (CPEB3) regulates mRNA translation and has roles in various cellular functions.
  • The specific function of CPEB3 in bovine cumulus cells remains largely unknown.
  • Cumulus cells are crucial for oocyte maturation and fertilization.

Purpose of the Study:

  • To investigate the role of CPEB3 in regulating the biological and physiological functions of bovine cumulus cells.
  • To determine the effects of CPEB3 inhibition on cell cycle, proliferation, and apoptosis in these cells.

Main Methods:

  • Small interfering RNA (siRNA) was used to inhibit CPEB3 mRNA expression in bovine cumulus cells.
  • Cell cycle progression, proliferation, and apoptosis were assessed.
  • Intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential, and expression of key genes (e.g., cell cycle regulators, expansion-related genes) were examined.

Main Results:

  • CPEB3 inhibition led to an abnormal cell cycle with increased S and G2/M phases.
  • Cell proliferation was enhanced, while apoptosis rates decreased following CPEB3 inhibition.
  • Changes observed were associated with decreased intracellular ROS, increased mitochondrial membrane potential, and altered expression of cell cycle and expansion-related genes (e.g., CCNA, CCNB, CDK1, HAS2, PTGS2).

Conclusions:

  • CPEB3 plays a significant role in regulating the biological and physiological functions of bovine cumulus cells.
  • Modulation of CPEB3 affects cell cycle dynamics, proliferation, and apoptosis, likely through ROS and mitochondrial pathways.
  • These findings provide insights into the molecular mechanisms governing cumulus cell function during reproductive processes.
Keywords:
CPEB3ROSapoptosisbovine cumulus cellproliferation

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