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Related Experiment Video

Updated: Dec 15, 2025

Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials

Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials

Published on: August 13, 2019

Osterix-mCherry Expression Allows for Early Bone Detection in a Calvarial Defect Model.

Sara E Strecker¹, Shimon Unterman¹, Lyndon F Charles¹

¹Massachusetts Institute of Technology, Institute for Medical Engineering and Science, 45 Carleton Street, E25-438, Cambridge, MA, 02139, USA.

Advanced Biosystems

|July 11, 2020

Summary

This summary is machine-generated.

Tracking Osterix, a key bone formation gene, using fluorescence allows early prediction of bone repair success. This method enables rapid screening of bone-healing drugs and delivery systems within weeks, accelerating therapeutic development.

Keywords:

BMP-2 Osterix bone regeneration imaging nanocomposite scaffold

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Last Updated: Dec 15, 2025

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Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry

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Published on: March 14, 2020

Area of Science:

Biomaterials Science
Regenerative Medicine
Molecular Biology

Background:

Bone repair involves complex cellular recruitment and mineralization, making treatment assessment lengthy.
Current methods for evaluating bone formation and treatment efficacy are time-consuming, often requiring months for mineralization assessment.

Purpose of the Study:

To evaluate the utility of a fluorescently tagged Osterix transcription factor as an early, noninvasive predictive measure of bone formation.
To assess the correlation between Osterix expression levels and subsequent bone healing following treatment with recombinant human Bone-Morphogenetic-Protein 2 (rhBMP-2).

Main Methods:

Development and utilization of an Osterix-mCherry mouse model for noninvasive tracking of Osterix expression.
Application of a calvarial defect model in mice treated with rhBMP-2 delivered via a nanocomposite hydrogel and a collagen sponge.
Monitoring of cherry-fluorescent protein signal as a proxy for Osterix activity and subsequent bone formation.

Main Results:

Fluorescent protein (cherry) signal, indicative of Osterix activity, was detectable as early as two weeks post-treatment.
Osterix intensity demonstrated a positive correlation with the extent of subsequent bone formation.
Distinct release kinetics of the delivery materials influenced the observed fluorescence patterns.

Conclusions:

Fluorescently tagged Osterix serves as a rapid and predictive biomarker for assessing bone formation and treatment efficacy.
This noninvasive tracking method can significantly accelerate the screening of osteogenic drugs and the optimization of biomaterial delivery platforms for bone regeneration.