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Updated: Dec 15, 2025

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Membrane Poration Mechanisms at the Cell-Nanostructure Interface.

Michele Dipalo1, Valeria Caprettini1, Giulia Bruno1,2

  • 1Istituto Italiano di Tecnologia, Genoa, 16163, Italy.

Advanced Biosystems
|July 11, 2020
PubMed
Summary

Investigating 3D nanostructures for cell poration reveals distinct membrane dynamics between optoacoustic- and electro-poration methods. This study details cell membrane responses to nanostructure-mediated poration for improved intracellular access applications.

Keywords:
electrophysiologyelectroporationmembrane deformationplasmonic optoporationscanning electron microscopy

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Area of Science:

  • Biotechnology and Nanomedicine
  • Cellular Biophysics

Background:

  • 3D vertical nanostructures enable cell interfacing and intracellular access, crucial for applications like measuring membrane potential.
  • Understanding cell membrane responses post-poration is vital for assessing nanostructure-mediated poration techniques.
  • Previous studies lacked detailed comparisons of membrane dynamics following different poration events.

Purpose of the Study:

  • To provide new insights into cell membrane dynamics during nanostructure-mediated poration.
  • To compare the effects of optoacoustic- and electro-poration on cell membranes using identical 3D nanostructures.
  • To assess cell condition and membrane conformation after poration events.

Main Methods:

  • Utilized 3D vertical nanostructures for cell membrane poration.
  • Employed optoacoustic- and electro-poration as distinct poration mechanisms.
  • Conducted detailed experimental analyses including electrical recordings and membrane conformation studies.

Main Results:

  • Observed and detailed differences in membrane dynamics between optoacoustic- and electro-poration.
  • Provided a comprehensive overview of the poration processes.
  • Characterized membrane conformation changes induced by the two poration methods.

Conclusions:

  • The study elucidates distinct membrane dynamics associated with different poration methods mediated by 3D nanostructures.
  • Findings offer a deeper understanding of cell membrane behavior post-nanostructure interaction.
  • Results contribute to the advancement of nanostructure-based intracellular interfacing technologies.