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Updated: Dec 15, 2025

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Published on: August 5, 2021
TEM observation of inorganic substances distributed in gel materials for medical devices using ultra-thin
Eri Ito1,2, Hiroshi Takase3, Katsuhiro Yamamoto1
1Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan.
Abstract:
In this study, a method was developed for examining the distribution pattern of inorganic substances dispersed in hydrogel-filled medical devices. Transmission electron microscopy (TEM) using ultra-thin cryosectioning (owing to hydrogel's water content) was performed on contact lenses with an iris pattern in which the distribution pattern of inorganic pigments was problematic. We confirmed the depth and distribution pattern of pigments in the hydrogel. The results indicated that ultra-thin cryosectioning with TEM was effective for inspecting the distribution of inorganic substances in hydrogel-filled devices.
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