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Development of a Multilocus Sequence Typing Scheme for Giardia intestinalis
Adriana Higuera1, Marina Muñoz1, Myriam Consuelo López2
1Grupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá 111211, Colombia.
Genes
|July 12, 2020
Summary
This study evaluated new genetic markers to understand Giardia intestinalis diversity. New markers effectively identified Giardia assemblages and revealed genetic recombination, aiding in parasite genetics research.
Area of Science:
- Microbiology
- Parasitology
- Genetics
Background:
- Giardia intestinalis is a common human intestinal protozoan parasite.
- It is classified into 8 assemblages (A-H), with genotyping traditionally relying on specific genes.
- Assessing genetic diversity and structure is crucial for understanding Giardia epidemiology.
Purpose of the Study:
- To evaluate novel genetic markers for studying Giardia intestinalis diversity and intra-taxa genetic structure.
- To analyze genetic variation using both in silico genome data and DNA from clinical stool samples.
Main Methods:
- Analysis of nine constitutive genes in 80 Giardia intestinalis complete genome sequences.
- Genotyping of 24 stool samples from Colombia using the selected genetic markers.
- Evaluation of allelic diversity and phylogenetic reconstructions to identify assemblages.
- Population structure analysis to assess genetic differentiation.
Main Results:
- The nine novel markers successfully discriminated up to 53 alleles in Giardia intestinalis.
- Phylogenetic analysis identified assemblages AI, AII, and B.
- Evidence of intra- and inter-assemblage recombination events was detected.
- Population structure analysis confirmed genetic differentiation among the studied assemblages.
Conclusions:
- The evaluated genetic markers are valuable tools for assessing Giardia intestinalis diversity and genetic structure.
- These markers can aid in identifying specific assemblages and understanding recombination patterns.
- The findings contribute to a better understanding of Giardia genetics and population dynamics.
