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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

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Related Experiment Video

Updated: Dec 15, 2025

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Published on: March 8, 2024

Modeling endodermal organ development and diseases using human pluripotent stem cell-derived organoids.

Fong Cheng Pan¹, Todd Evans¹, Shuibing Chen¹

¹Department of Surgery, Weill Cornell Medical College, New York, NY 10065, USA.

Journal of Molecular Cell Biology

|July 12, 2020

Summary

This summary is machine-generated.

Human pluripotent stem cells (hPSCs) and 3D organoid technology enable modeling of gastrointestinal development and diseases. This review covers hPSC-derived organoids for stomach, liver, pancreas, and intestines, highlighting future integration with genome editing and bioengineering.

Keywords:

disease modeling endoderm gastrointestinal development human pluripotent stem cells

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Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Published on: September 15, 2021

Area of Science:

Stem cell biology
Developmental biology
Gastroenterology

Background:

Directed differentiation protocols for human pluripotent stem cells (hPSCs) have advanced significantly.
3D organoid technology allows for the creation of complex, tissue-specific structures.
Endoderm-derived organoids are crucial for in vitro modeling of human gastrointestinal development and diseases.

Purpose of the Study:

To review current strategies for generating hPSC-derived endodermal organoids.
To discuss the application of these organoids in modeling human diseases.
To explore the integration of complementary technologies for enhanced modeling platforms.

Main Methods:

Directed differentiation of hPSCs into specific endodermal lineages.
3D organoid culture techniques for stomach, liver, pancreas, small intestine, and colon.
Literature review of existing methodologies and applications.

Main Results:

Established protocols exist for generating various hPSC-derived endodermal organoids.
These organoids serve as valuable models for human gastrointestinal development and disease.
Current limitations and future directions for improving these models were identified.

Conclusions:

hPSC-derived organoids represent a powerful tool for studying human gastrointestinal development and disease.
Integration with genome editing and bioengineering will further enhance their utility.
These advanced models hold significant promise for regenerative medicine and therapeutic development.