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Modulating activity of B lymphocytes on haemopoiesis (II)--the purification of proliferation amplifying factor (PAF)

Z J Yao1, F Z Jiang, Y Li

  • 1Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.

Scientia Sinica. Series B, Chemical, Biological, Agricultural, Medical & Earth Sciences
|June 1, 1988
PubMed

In recent years we have done a series of observations on the haemopoietic modulation effects of lymphocytes. Now we report here, for the first time, the purification of a modulating factor (proliferation amplifying factor, PAE) with affinity chromatography and HPLC from supernatant conditioned by a lymphoma cell line M12.4.1. RAF exhibits on SDS-PAGE only one band, the molecular weight is about 31,000, the specific activity is 20,000 times that in the conditioned medium. It may provide a direct evidence that the B lymphocyte also possesses some haemopoietic modulation effects.

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