Design and optimization of bioreactor to boost carbon dioxide assimilation in RuBisCo-equipped Escherichia coli

Global warming is a surging issue that has provoked the demand of green process to mitigate carbon dioxide. In this context, RuBisCo-equipped Escherichia coli has first developed and evaluated the CO₂-assimiliable capability based on the mass balance in three devices: Flask-based in CO₂ incubator (FIC), two-layered device (TLD) and CO₂ bubbling device (CBD) systematically. With the forced diffusion of 5% CO₂ in CBD, which confers an efficient attack of CO₂ to RuBisCo, the CO₂ assimilation increased from -5.03 to -2.63 g-CO₂/g-DCW. Furthermore, boosted CO₂ assimilation ability was observed by co-expression of GroELS chaperone with 71% reduction on CO₂ release. By DNA sequencing and tandem MS/MS analysis, the toxicity of RuBisCo and PRK was identified to interfere the sugar metabolism and energy producing, while the cell morphology was changed and observed in RuBisCo-equipped E. coli. Our study provides a new perspective of higher CO₂ assimilation for sustainable to eco-friendly green bioprocess.