Related Experiment Video
Updated: Dec 15, 2025
10:01
Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
18.6K
Development of a simultaneous quantification method for ten trichothecenes including deoxynivalenol-3-glucoside in
Masayo Nomura1, Kenji Shidara2, Iyo Yasuda3
1Food and Agricultural Materials Inspection Center, Saitama Shintoshin National Government Building, Kensato Building, Shintoshin 2-1, Chuo-Ku, Saitama-Shi, Saitama, 330-9731, Japan. masayo_nomura336@famic.go.jp.
Mycotoxin Research
|July 13, 2020
Summary
A new liquid chromatography-tandem mass spectrometry method accurately quantifies ten trichothecenes in animal feed. This method improves extraction and reduces signal interference for reliable mycotoxin analysis.
Area of Science:
- Analytical Chemistry
- Food Safety
- Veterinary Toxicology
Background:
- Trichothecenes are mycotoxins commonly found in animal feed, posing risks to animal health and food safety.
- Accurate and simultaneous quantification of multiple trichothecenes is crucial for risk assessment and regulatory compliance.
- Existing analytical methods may face challenges with extraction efficiency, matrix effects, and simultaneous detection of diverse trichothecene types.
Purpose of the Study:
- To develop and validate a sensitive analytical method for the simultaneous determination of ten trichothecenes (types A and B) in various animal feed matrices.
- To optimize extraction and clean-up procedures to minimize matrix interferences and enhance detection sensitivity.
- To assess the method's performance, including recovery, repeatability, limit of quantification, and limit of detection.
Main Methods:
- Simultaneous extraction of mycotoxins from feed samples using aqueous acetonitrile.
- Purification using a multifunctional clean-up column and a phospholipid removal column to mitigate ionization enhancement.
- Analysis by liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCI-MS/MS).
Main Results:
- The developed LC-APCI-MS/MS method successfully quantified ten trichothecenes in diverse feed matrices, including maize, barley, soybean meal, rapeseed meal, and formula feeds.
- Spike and recovery tests demonstrated good accuracy, with mean recovery values ranging from 70.6% to 119% and relative standard deviations below 17%.
- The method achieved sensitive detection limits, with limits of quantification as low as 5 μg/kg for certain mycotoxins.
Conclusions:
- The established analytical method provides a reliable and efficient approach for the simultaneous quantification of multiple trichothecenes in animal feed.
- The optimized clean-up strategy effectively addresses ionization enhancement, improving the accuracy of mycotoxin determination.
- This method supports enhanced monitoring of feed safety and contributes to safeguarding animal health and the food supply chain.
