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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

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Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
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Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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Related Experiment Video

Updated: Dec 15, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

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Including Digital Sequence Data in the Nagoya Protocol Can Promote Data Sharing.

Jon Ambler1, Alpha Ahmadou Diallo2, Peter K Dearden3

  • 1Computational Biology Division, University of Cape Town, Cape Town, South Africa; Wellcome Centre for Infectious Disease Research in Africa, University of Cape Town, Cape Town, South Africa.

Trends in Biotechnology
|July 14, 2020
PubMed
Summary
This summary is machine-generated.

Formalized benefit-sharing for digital sequence information (DSI) can enhance research collaboration. Equitable agreements ensure fair benefit distribution, fostering trust and data sharing for global scientific advancement.

Keywords:
Nagoya Protocolbenefit sharingbiological datadata accessdata sharingdigital sequence information

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Novel Sequence Discovery by Subtractive Genomics
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Area of Science:

  • Biodiversity Law and Policy
  • Bioinformatics
  • Conservation Science

Background:

  • The Nagoya Protocol (NP) governs benefit-sharing from biological resources under the Convention on Biological Diversity (CBD).
  • Concerns exist that applying NP benefit-sharing to digital sequence information (DSI) may impede scientific research.
  • Current frameworks address physical samples but not necessarily the data derived from them.

Purpose of the Study:

  • To propose a framework for formalised benefit-sharing of digital sequence information (DSI).
  • To demonstrate how equitable benefit-sharing can foster research participation and data sharing.
  • To address concerns regarding the impact of NP on DSI research.

Main Methods:

  • Analysis of the Nagoya Protocol and its implications for digital sequence information (DSI).
  • Development of a conceptual model for equitable benefit-sharing agreements.
  • Examination of three case studies illustrating practical applications of benefit-sharing.

Main Results:

  • Formalised benefit-sharing for DSI can increase willingness to participate in research and share data.
  • Equitable collaboration between sample providers and researchers can be fostered through clear agreements.
  • Preventing exploitative practices is crucial for building trust and long-term partnerships.

Conclusions:

  • Equitable benefit-sharing agreements for DSI can enhance global research collaborations.
  • Implementing fair practices ensures that those providing biological samples and associated knowledge benefit.
  • This approach supports sustainable research and equitable outcomes in biodiversity research.