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Related Experiment Video

Updated: Dec 15, 2025

Human Blastocyst Biopsy and Vitrification
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An effective method for trophectoderm biopsy using mechanical blunt dissection: a step-by-step demonstration.

Dalei Yang1, Di Feng1, Yingzhuo Gao1

  • 1Center of Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.

Fertility and Sterility
|July 14, 2020
PubMed
Summary

This study presents a mechanical blunt dissection (MBD) technique for trophectoderm biopsy, achieving a 95.4% success rate in blastocyst biopsies and 100% survival post-warming. The MBD method offers advantages for various blastocyst stages and reduces thermal damage.

Keywords:
Artificial shrinkageblastocystpreimplantation genetic testingtrophectoderm biopsy

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Area of Science:

  • Reproductive biology
  • Embryology
  • Assisted reproductive technology

Background:

  • Trophectoderm biopsy is crucial for preimplantation genetic testing (PGT).
  • Standard biopsy methods may have limitations in handling diverse blastocyst stages and characteristics.
  • Minimizing thermal damage during biopsy is essential for embryo viability.

Purpose of the Study:

  • To introduce and evaluate an effective trophectoderm biopsy approach using mechanical blunt dissection (MBD).
  • To demonstrate the technique's applicability across different blastocyst stages and morphologies.
  • To assess the success and survival rates of blastocysts biopsied with the MBD method.

Main Methods:

  • Micromanipulation techniques including artificial shrinkage and zona pellucida drilling.
  • Trophectoderm cell aspiration using specific biopsy pipette sizes (20-27 μm).
  • Detachment of cells via laser pulse combined with MBD, utilizing pipette rims for dissection.

Main Results:

  • A high successful biopsy rate of 95.4% (n=1,872) was achieved.
  • Excellent post-warming survival rate of 100% (n=440).
  • The MBD technique demonstrated advantages in handling various blastocyst types and potentially reduced thermal damage compared to laser-only methods.

Conclusions:

  • The described MBD approach is effective and versatile for trophectoderm biopsy.
  • The technique simplifies the biopsy process and can be widely applied in clinical settings.
  • This method offers a reliable option for PGT, ensuring high success and survival rates.