A Mini-review of the Computational Methods Used in Identifying RNA 5-Methylcytosine Sites

RNA 5-methylcytosine (m⁵C) is one of the pillars of post-transcriptional modification (PTCM). A growing body of evidence suggests that m⁵C plays a vital role in RNA metabolism. Accurate localization of RNA m⁵C sites in tissue cells is the premise and basis for the in-depth understanding of the functions of m⁵C. However, the main experimental methods of detecting m⁵C sites are limited to varying degrees. Establishing a computational model to predict modification sites is an excellent complement to wet experiments for identifying m⁵C sites. In this review, we summarized some available m⁵C predictors and discussed the characteristics of these methods.