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Updated: Dec 15, 2025
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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
Published on: September 13, 2018
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BORA regulates cell proliferation and migration in bladder cancer
Songtao Cheng1,2,3, Tianchen Peng1,3, Xiaolu Zhu4
1Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Cancer Cell International
|July 14, 2020
Summary
BORA, a kinase activator, is upregulated in bladder cancer (BCa) and drives tumor growth and cell cycle progression. Inhibiting BORA offers a potential therapeutic strategy for BCa.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Bladder cancer (BCa) incidence is rising, with limited new therapeutic options beyond chemotherapy for decades.
- There is an urgent need for novel therapeutic targets and biomarkers in BCa treatment.
Purpose of the Study:
- To investigate the role of BORA, an Aurora A kinase activator, in bladder cancer progression.
- To explore BORA as a potential therapeutic target and biomarker for BCa.
Main Methods:
- Established BORA knockdown and overexpression cell models for in vitro studies.
- Utilized xenograft and pulmonary metastasis mouse models for in vivo validation.
- Analyzed cell cycle, proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) pathways.
Main Results:
- BORA expression was significantly upregulated in human BCa tissues at both transcriptional and translational levels.
- BORA knockdown induced G2/M cell cycle arrest and inhibited proliferation, migration, and invasion.
- BORA overexpression had the opposite effect, and alterations in PLK1-CDC25C-CDK1 and EMT pathways were observed.
Conclusions:
- BORA plays a critical role in regulating BCa cell cycle, growth, and motility via the EMT pathway.
- BORA represents a promising novel biomarker and potential therapeutic target for bladder cancer.
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