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Updated: Dec 15, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
Sirtuin 7 Promotes Mesenchymal to Epithelial Transition by β-Catenin Redistribution and Stabilization
Shashi Kiran1,2, Manjari Kiran2,3, Gayatri Ramakrishna4
1Department of Biochemistry, University of Hyderabad, Hyderabad, India.
Abstract:
SIRT7 belongs to the family of "NAD+ dependent deacetylases" called Sirtuins. In the present work we report a novel role of SIRT7 in regulating cellular polarity. SIRT7 overexpression in immortalized mouse fibroblasts (NIH3T3) induced epithelial transition. This transition was accompanied by typical N- to E- cadherin transition, stabilization of β-catenin, and the downregulation of transcription factors responsible for maintenance of mesenchymal phenotype (Snail, Slug, and Zeb1). Interestingly, a subpopulation of cells overexpressing SIRT7 exhibited an intermediate stage between mesenchymal and epithelial characters. Transformed epithelial cells showed a loss of heterochromatisation as evidenced by a loss of HP1α and H3K9 dimethylation staining. In conclusion, we report a role of SIRT7 in mesenchymal cells, which may have implications for health and disease.
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