Recent Development in Ligation Methods for Glycopeptide and Glycoprotein Synthesis
Junjie Desmond Lin1, Xue-Wei Liu1
1Division of Chemistry and Biological Chemistry School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore, 637371, Singapore.
Chemistry, an Asian Journal
|July 14, 2020
Summary
Producing pure glycoproteins is challenging due to heterogeneous carbohydrate structures. This review covers methods for preparing and ligating glycopeptides, highlighting chemical synthesis as a future direction for homogenous glycoprotein production.
Area of Science:
- Biochemistry
- Organic Chemistry
- Glycoscience
Background:
- Glycoproteins are crucial for biological processes, but their functions are poorly understood.
- Heterogeneous carbohydrate structures on glycoproteins hinder research.
- Isolation of pure glycoforms is difficult with current recombinant and chromatographic methods.
Purpose of the Study:
- To review methods for preparing and ligating glycopeptides.
- To discuss the importance of chemical synthesis for producing homogenous glycoproteins.
Main Methods:
- Review of selected ligation and glycosylation techniques.
- Discussion of chemical synthesis strategies for glycoproteins.
Main Results:
- Current methods struggle to isolate pure glycoforms.
- Various ligation and glycosylation techniques have been developed.
- Chemical synthesis offers a promising route to homogenous glycoproteins.
Conclusions:
- Homogenous glycoproteins are essential for understanding their biochemical functions.
- Advanced chemical synthesis methods are needed for future glycoprotein research.
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