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Published on: November 21, 2013
LINE-1 hypomethylation is associated with poor risperidone response in a first episode of psychosis cohort
Diogo Ferri Marques1,2,3, Vanessa Kiyomi Ota2,3, Marcos Leite Santoro1,2,3
1Department of Psychiatry, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil.
Abstract:
Aim: We investigated the DNA methylation profile over LINE-1 in antipsychotic-naive, first-episode psychosis-patients (n = 69) before and after 2 months of risperidone treatment and in healthy controls (n = 62). Materials & methods: Patients were evaluated using standardized scales and classified as responders and nonresponders. DNA from blood was bisulfite converted and LINE-1 fragments were amplified and pyrosequencing was performed. Results: Lower LINE-1 methylation was observed in antipsychotic-naive first-episode psychosis patients than in healthy controls. Lower DNA methylation levels before treatment were associated with poor risperidone responses. A positive correlation was observed between LINE-1 methylation levels and positive symptoms response. Conclusion: Our study brings new insight regarding how epigenomic studies and clinical correlation studies can supplement psychosis treatment.
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