Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hypercholesterolemia after conversion to sirolimus as primary immunosuppression and cardiac allograft vasculopathy in heart transplant recipients.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2018

Same author

Ubiquitous yet unseen: microvascular endothelial dysfunction beyond the heart.

European heart journal·2018

Same author

Cardiorenal biomarkers: one step closer.

Journal of laboratory and precision medicine·2018

Same author

Microvascular obstruction in non-infarct related coronary arteries is an independent predictor of major adverse cardiovascular events in patients with ST segment-elevation myocardial infarction.

International journal of cardiology·2018

Same author

Coronary endothelial function and spontaneous coronary artery dissection.

European heart journal. Acute cardiovascular care·2018

Same author

Enhancing Mitochondrial Health to Treat Hypertension.

Current hypertension reports·2018

Search research articles

Related Experiment Video

Updated: Dec 15, 2025

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

Published on: August 7, 2017

Mesenchymal Stem/Stromal Cells and their Extracellular Vesicle Progeny Decrease Injury in Poststenotic Swine Kidney

Yu Zhao^1,2, Xiangyang Zhu², Lei Zhang^2,3

¹Institute of Nephrology, Zhong Da Hospital, Southeast University, School of Medicine, Nanjing, China.

Stem Cells and Development

|July 14, 2020

Summary

Mesenchymal stem cells (MSCs) and their extracellular vesicles (EVs) both improve kidney function and reduce injury in a pig model of chronic kidney disease. MSCs better preserved microvasculature, while EVs enhanced cellular integrity.

Keywords:

extracellular vesicles mesenchymal stem cells renal artery stenosis

More Related Videos

Establishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment

Establishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment

Published on: May 25, 2020

A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery Stenosis

A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery Stenosis

Published on: October 26, 2020

Related Experiment Videos

Last Updated: Dec 15, 2025

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

Published on: August 7, 2017

Establishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment

Establishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment

Published on: May 25, 2020

A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery Stenosis

A Modified Two Kidney One Clip Mouse Model of Renin Regulation in Renal Artery Stenosis

Published on: October 26, 2020

Area of Science:

Nephrology
Regenerative Medicine
Cell Biology

Background:

Chronic kidney disease (CKD) prevalence is rising, necessitating novel therapeutic strategies.
Mesenchymal stem/stromal cells (MSCs) and their derived extracellular vesicles (EVs) show promise in tissue repair.
The comparative efficacy and distinct mechanisms of MSCs versus EVs in renal protection remain to be fully elucidated.

Purpose of the Study:

To test the hypothesis that EVs are as effective as MSCs in protecting the stenotic kidney but target different injury pathways.
To evaluate the therapeutic potential of adipose tissue-derived MSCs and their EVs in a preclinical pig model of metabolic syndrome and renal artery stenosis (MetS + RAS).

Main Methods:

A pig model of MetS + RAS was established, with subsequent intrarenal delivery of autologous MSCs or EVs.
Renal function was assessed in vivo using CT and MRI, alongside ex vivo histopathology and molecular marker analysis (necroptosis, inflammation, growth factors).
Lean pigs and sham RAS served as control groups.

Main Results:

Both MSCs and EVs improved stenotic kidney function, decreasing hypoxia, fibrosis, and apoptosis.
MSCs showed a slight advantage in preserving microvascular density and reducing inflammation.
EVs demonstrated a more significant upregulation of growth factors and a greater reduction in necroptosis.

Conclusions:

Adipose tissue-derived MSCs and their EVs both ameliorate renal injury and improve function in MetS + RAS pigs through partially distinct mechanisms.
MSCs excel at preserving microcirculation, whereas EVs offer superior protection of renal cellular integrity.
These findings support further investigation into MSCs and EVs as a novel therapeutic approach for attenuating renal injury.