Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.3K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.2K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.2K
Reporter Genes02:11

Reporter Genes

12.6K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
12.6K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

25.4K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.4K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.7K
3.7K
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.4K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tuning Circular Dichroism and Circularly Polarised Luminescence in Single Crystals of a Perylene Diimide Macrocycle.

Angewandte Chemie (International ed. in English)·2026
Same author

Conformational switching modulates excited-state pathways in a cofacial perylene dimer.

Chemical science·2026
Same author

Correction to "DNA-Programmable Protein Degradation: Dynamic Control of Proteolysis-Targeting Chimera Activity via DNA Hybridization and Strand Displacement".

JACS Au·2026
Same author

Chirally locked and dynamic bis-perylene diimide macrocycles with multiple sources of chirality.

Communications chemistry·2026
Same author

The effect of PEGylation on surface tethering of liposomes via DNA nanotechnology.

Journal of lipid research·2025
Same author

Magnetic activation of spherical nucleic acids enables the remote control of synthetic cells.

Nature chemistry·2025
Same journal

TDP-43 proteinopathy as a biomarker and therapeutic target in amyotrophic lateral sclerosis.

Biochemical Society transactions·2026
Same journal

Advancing the monitoring of organelle contact sites in vitro and in vivo.

Biochemical Society transactions·2026
Same journal

Mechanisms influencing transient cytoplasmic protein targeting to intracellular lipid droplets.

Biochemical Society transactions·2026
Same journal

Replication associated nuclear DNA mismatch repair across kingdoms.

Biochemical Society transactions·2026
Same journal

Phosphatases of regenerating liver downregulate PTEN to promote tumorigenesis.

Biochemical Society transactions·2026
Same journal

Implications of Rho GTPase signaling in cancer immunotherapy.

Biochemical Society transactions·2026
See all related articles

Related Experiment Video

Updated: Dec 15, 2025

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

937

Controlling gene expression with light: a multidisciplinary endeavour.

Denis Hartmann1, Jefferson M Smith1, Giacomo Mazzotti1

  • 1Chemistry Research Laboratory, University of Oxford, Oxford OX1 3TA, U.K.

Biochemical Society Transactions
|July 14, 2020
PubMed
Summary
This summary is machine-generated.

Researchers explore light-controlled gene expression, using photocages and light-sensitive proteins. Recent advances utilize longer wavelengths for safer, deeper biological control in biotechnology.

Keywords:
biochemical techniques and resourcesbiotechnologygene expression and regulation

More Related Videos

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.7K
Light-Controlled Fermentations for Microbial Chemical and Protein Production
08:37

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

4.5K

Related Experiment Videos

Last Updated: Dec 15, 2025

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

937
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.7K
Light-Controlled Fermentations for Microbial Chemical and Protein Production
08:37

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

4.5K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Synthetic Biology

Background:

  • Gene expression is a fundamental biological process.
  • Light offers precise spatiotemporal control over biological systems.
  • Existing methods for light-controlled gene expression have limitations.

Purpose of the Study:

  • To review chemical and biological methods for light-controlled gene expression.
  • To highlight key themes and applications in this field.
  • To discuss the shift towards longer wavelengths for improved safety and penetration.

Main Methods:

  • Modification of biomolecules (nucleic acids, proteins) with photocages.
  • Engineering of gene expression machinery with naturally light-sensitive proteins.
  • Utilizing various wavelengths of light, including longer wavelengths.

Main Results:

  • A diverse array of techniques exists for controlling transcription and translation with light.
  • Photocages and engineered light-sensitive proteins are key tools.
  • Longer wavelengths (e.g., visible light) offer advantages over ultraviolet light.

Conclusions:

  • Light-controlled gene expression is a rapidly advancing field with broad applications.
  • The development of longer-wavelength systems enhances safety and tissue penetration.
  • This technology holds significant promise for cell-free and in vivo biotechnology.