Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

6.5K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.5K
Master Transcription Regulators02:23

Master Transcription Regulators

7.6K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K
Transcription Factors02:16

Transcription Factors

81.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
81.9K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.3K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

7.0K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.0K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.2K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The DBD-α4 helix of EWSR1::FLI1 is required for GGAA microsatellite binding that underlies genome regulation in Ewing sarcoma.

eLife·2026
Same author

A scalable, multi-resolution consensus clustering approach for prioritizing robust signals from high-throughput screens.

Briefings in bioinformatics·2026
Same author

HES3-dependent regulatory functions in development and fusion-positive rhabdomyosarcoma.

bioRxiv : the preprint server for biology·2026
Same author

Identifying therapeutic targets in low-grade serous ovarian carcinomas with no specific molecular profile.

The Journal of pathology·2026
Same author

Differential Preclinical Efficacy of Combined CDK4/6 and MEK Inhibition in Low-Grade Serous Ovarian Carcinoma Based on <i>KRAS/NF1</i> Mutational Status.

International journal of molecular sciences·2026
Same author

Comparative modes of chromatin engagement by PAX::FOXO1 fusions in rhabdomyosarcoma.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Dec 15, 2025

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.0K

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic

Iftekhar A Showpnil1, Kyle R Miller2, Cenny Taslim2

  • 1Center for Childhood Cancer and Blood Diseases, Abigail Wexner Research Institute at Nationwide Children's Hospital; Molecular, Cellular, and Developmental Biology Program, The Ohio State University.

Journal of Visualized Experiments : Jove
|July 14, 2020
PubMed
Summary

This study reveals key structural features of the EWSR1 intrinsically disordered domain (IDD) crucial for EWS/FLI oncogenic function in Ewing sarcoma. RNA-sequencing identified novel target genes essential for cancer development.

More Related Videos

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.3K
CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

13.0K

Related Experiment Videos

Last Updated: Dec 15, 2025

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.0K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
11:32

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

7.3K
CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

13.0K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Many cancers involve oncogenic fusion transcription factors, often containing intrinsically disordered domains (IDDs).
  • The EWSR1 N-terminal domain is an IDD found in critical oncogenic fusions like EWS/FLI.
  • Targeting these factors requires understanding the role of IDDs in their function.

Purpose of the Study:

  • To investigate the structural features of the EWSR1 EWS domain essential for the transcriptional function of EWS/FLI in Ewing sarcoma.
  • To identify novel EWS/FLI target genes critical for Ewing sarcoma oncogenesis.

Main Methods:

  • Utilized shRNA to deplete endogenous EWS/FLI in Ewing sarcoma cells.
  • Employed RNA-sequencing to analyze transcriptomes of cells expressing various EWS-mutant constructs.
  • Integrated transcriptomic data with existing information on EWS/FLI DNA binding and localization.

Main Results:

  • Identified specific structural features within the EWS domain critical for EWS/FLI oncogenic activity.
  • Defined a novel set of EWS/FLI target genes essential for Ewing sarcoma.
  • Demonstrated RNA-sequencing's utility in mapping structure-function relationships of IDDs in oncogenic transcription factors.

Conclusions:

  • The study elucidates the structure-function relationship of the EWSR1 IDD in EWS/FLI.
  • Findings provide insights into Ewing sarcoma pathogenesis and potential therapeutic strategies targeting EWS/FLI.
  • RNA-sequencing is a valuable tool for dissecting the role of IDDs in cancer.