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Published on: October 4, 2019
Identification of Mir-9 in Glioma Diagnosis and Prognosis
Background:
Mir-9 was recognized as a potential tumor suppressor gene or oncogene in varies of diseases; however, its roles in glioma have not been investigated. Our study was to identify the mRNA expression of mir-9 gene in glioma and correlate abnormal versions of microRNAs with clinicopathological features and investigate the impact of mir-9 in glioma prognosis.
Methods:
Seventy-one glioma samples, which included 22 grade II, 24 grade III, and 25 glioblastoma tumors of previously untreated patients who underwent surgical excision at Qing Hai Province People's Hospital from 2011 to 2014, were included. In addition, 2 epilepsy patients with normal brain tissue were included as a control group. The expression of mir-9 was examined by RT-PCR in formalin-fixed paraffin embedded primary tissue specimens. The clinicopathologic features, the survival rate of glioma patients were also discussed. The RNA expression of mir-9 and glioma prognosis was evaluated using a Chi-square test, Cox regression model, and GraphPad Prism survival curve analysis.
Results:
There were 16, 20, 21 cases which showed high expression of mir-9 in grade II and III gliomas and glio-blastoma, 16/22 (72.7%), 20/24 (83.3%), 21/25 (84.0%), respectively. The expression of mir-9 was correlated with the grade of glioma. The mir-9 RNA expression in glioblastoma were higher than grade II and III gliomas (p < 0.05). The higher the grade, the higher the expression, and the difference was significant (p < 0.05), while it was not correlated with patient nationality, gender, or location (p > 0.05). Univariate analysis determined that high expression of mir-9, grade, chemotherapy, radiation therapy, and patient onset age were associated with glioma patient prognosis (p < 0.05).
Conclusions:
The expression of mir-9 plays a role in glioma progression, and may be used as a prognostic marker in glioma.
Insights
MicroRNA-9 (mir-9) expression increases with glioma grade and is associated with poorer prognosis. High mir-9 levels indicate a potential prognostic marker for glioma progression.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Genetics
Background:
- MicroRNA-9 (mir-9) has varied roles in disease, but its function in glioma remains unexplored.
- Investigating mir-9 expression is crucial for understanding glioma pathogenesis.
Purpose of the Study:
- To determine mir-9 mRNA expression levels in glioma tissues.
- To correlate mir-9 expression with clinicopathological features.
- To assess the impact of mir-9 on glioma prognosis.
Main Methods:
- RT-PCR analysis of mir-9 expression in 71 glioma samples (grades II, III, and glioblastoma) and 2 normal brain tissues.
- Correlation analysis with clinicopathological data and survival rates.
- Statistical evaluation using Chi-square, Cox regression, and survival curve analysis.
Main Results:
- Mir-9 expression was significantly higher in glioblastoma compared to lower-grade gliomas (p < 0.05).
- Increased mir-9 expression correlated positively with glioma grade.
- High mir-9 expression, tumor grade, and treatment modalities were significant prognostic factors (p < 0.05).
Conclusions:
- Mir-9 expression is implicated in glioma progression.
- Mir-9 may serve as a valuable prognostic biomarker for glioma patients.
