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Hematon: a multicellular functional unit in primary hematopoiesis.

I Blazsek1, J L Misset, M Comisso

  • 1ICIG and SMSIT, Université Paris-Sud, Villejuif, France.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|January 1, 1988
PubMed
Summary
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Multicellular spheroids, termed hematons, are abundant in healthy bone marrow and support blood cell production. Their absence in certain leukemias suggests a role in hematopoiesis and disease monitoring.

Area of Science:

  • Hematology
  • Cell Biology
  • Stem Cell Research

Background:

  • Bone marrow contains low-density multicellular spheroids (100-500 microns).
  • These spheroids feature a 3D network of adipocytes, mesenchymal cells, macrophages, and hematopoietic progenitor cells.
  • Spheroids show 2-5 fold higher progenitor cell abundance than whole bone marrow.

Purpose of the Study:

  • To identify and characterize a novel multicellular spheroid in bone marrow.
  • To propose this spheroid as the fundamental unit of primary hematopoiesis, naming it 'hematon'.
  • To investigate the correlation between hematon presence and homeostatic blood cell production in various conditions.

Main Methods:

  • Analysis of bone marrow aspirates from healthy donors and patients with myelodysplastic syndromes (MDPS), acute nonlymphocytic leukemias (ANLL), and chronic myeloid leukemia (CML).

Related Experiment Videos

  • Utilized monoclonal antibody markers (My 10, T 9), granulocyte-macrophage colony-forming cell (GM-CFC) assays, and cytological techniques.
  • Organotypic microcultures were used to assess the differentiation potential of the spheroids.

    • Main Results:

    • Hematons were present in normal bone marrow (19/25) but largely absent in MDPS (8/21), ANLL (3/22), and CML (2/28).
    • Hematons reappeared during hematological remission in MDPS and ANLL.
    • These findings indicate a positive correlation between hematon presence and homeostatic blood cell production.

    Conclusions:

    • The multicellular spheroid, or 'hematon', represents a fundamental unit of primary hematopoiesis.
    • Hematon presence is a potential biomarker for normal blood cell production.
    • Hematons may be reversibly dispersed in certain disease states, offering a unifying model for bone marrow cell function variability.